The adenosine triphosphate-based chemotherapy response assay (ATP-CRA) has the advantages of standardization, evaluability, reproducibility, and accuracy, and can be performed on relatively small numbers of tumor cells. A total of 43 patients were enrolled in the present study, and chemosensitivity tests were successfully performed in 40 (93.0%) of these patients. Twenty of the 40 received neoadjuvant chemotherapy or chemotherapy for metastatic breast cancer. The chemotherapy regimens used were doxorubicin plus docetaxel (n=9, 45.0%) or doxorubicin plus paclitaxel (n=11, 55.0%). Mean cell death rate, as determined by ATP-CRA, was lower in non-responders than in responders to therapy (P=0.012). Sensitivity, specificity, positive predictive value, negative predictive value, and diagnostic accuracy for ATP-CRA were 78.6%, 100%, 100%, 66.7%, and 85.0%, respectively. Diagnostic accuracy achieved by immunohistochemistry using estrogen receptor or progesterone receptor was lower than that achieved using ATP-CRA. Expression of p53, erb-B2, Ki67, Bcl-2, Bcl-xL, and annexin I was not significantly associated with response to chemotherapy. Our results show that ATP-CRA has high specificity and positive predictive value for predicting response to chemotherapy.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.breast.2007.06.001 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Highly sensitive surface-enhanced Raman scattering detection of adenosine triphosphate based on core-satellite assemblies.
Anal Methods
November 2017
Lab of Biosystem and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
As an important small molecule, adenosine triphosphate (ATP) plays an important role in the regulation of cell metabolism and supplies energy for various biochemical reactions in organisms. We herein developed a sensitive surface-enhanced Raman scattering (SERS) biosensor for highly specific detection of ATP using core-satellite assemblies. To construct the aptamer-based biosensor, a known ATP binding aptamer was divided into two segments.
View Article and Find Full Text PDFIdentification and validation of soft tissue sarcoma-specific transcriptomic model for predicting radioresistance.
Int J Radiat Biol
January 2025
Chungbuk National University College of Medicine, Cheongju, Republic of Korea.
Purpose: We aimed to identify the transcriptomic signatures of soft tissue sarcoma (STS) related to radioresistance and establish a model to predict radioresistance.
Materials And Methods: Nine STS cell lines were cultured. Adenosine triphosphate-based viability was determined 5 days after irradiation with 8 Gy of X-rays in a single fraction.
Fluorescence detection of adenosine triphosphate based on dimeric G-quadruplex.
Spectrochim Acta A Mol Biomol Spectrosc
February 2025
School of Life Sciences, Central South University, Changsha, China. Electronic address:
Article Synopsis
- ATP is a crucial energy carrier in living organisms, linked to various biological processes and diseases, making it an important biomarker for cell viability and food freshness.
- * The study presents a new ATP detection sensor that utilizes the fluorescence change of dimeric G-quadruplex (Di-G4) in the presence of ATP, showing a linear relationship between fluorescence and ATP concentrations.
- * When tested on apples, this novel method demonstrated reliable results when compared to traditional ATP assay kits, indicating its potential for practical applications.
Successful post-incomplete resection management of gastrointestinal stromal tumor using imatinib based on adenosine triphosphate-based tumor sensitivity assay in a dog.
Can Vet J
June 2024
KU Animal Cancer Center, Konkuk University Veterinary Medical Teaching Hospital, No. 120, Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea (Woo, Y-R Kim, S-W Kim, Choi, H-Y Yoon, Bae, K-A Yoon, J-H Kim); Department of Veterinary Internal Medicine (Woo, Y-R Kim, S-W Kim, J-H Kim) and Department of Veterinary Surgery (Choi, H-Y Yoon) and Department of Veterinary Biochemistry (Bae, K-A Yoon), College of Veterinary Medicine, Konkuk University, No. 120, Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea.
Gastrointestinal stromal tumors arising from gastric cardia are uncommon in dogs. A few studies have shown the effectiveness of tyrosine kinase inhibitors in the treatment of canine gastrointestinal stromal tumors, but no standardized protocols are currently available. An 11-year-old spayed female Maltese dog was diagnosed with a gastrointestinal stromal tumor using histopathological and immunohistochemical analyses.
View Article and Find Full Text PDFSynchronized Microbubble Photodynamic Activation to Disinfect Minimally Prepared Root Canals.
J Endod
February 2023
Dental Research Institute, Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada. Electronic address:
Introduction: The purpose of this study was to evaluate the antimicrobial efficacy of a novel irrigation strategy using synchronized microbubble photodynamic activation (SYMPA) in a minimally prepared single canal.
Methods: Single-canal mandibular incisors were inoculated with Enterococcus faecalis for 3 weeks and randomly allocated to 4 groups based on the irrigation protocols: (1) control (saline), (2) conventional needle irrigation (CI), (3) ultrasonic-assisted irrigation (UI), and (4) irrigation with SYMPA. The first 3 groups were instrumented to size 25.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!