AI Article Synopsis
- In a study on rubella antibodies from vaccinated adults, researchers found that using heat-denatured blocking proteins in the sample dilution buffer lowered the non-specific binding of IgG.
- This change in the ELISA technique improved the accuracy of the test by minimizing the chances of non-specific interactions.
- The findings indicate that modifying the standard ELISA could lead to fewer false positive results in antibody testing.
Article Abstract
During the analysis of rubella-specific antibodies in sera from adult vaccinees, it was observed that the inclusion of heat-denatured blocking proteins in the sample dilution buffer substantially reduced non-specific binding of serum IgG to microtitre plates. Results suggest that this modification to standard ELISA technique may reduce the incidence of false positive results in these assays.
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| http://dx.doi.org/10.1016/0022-1759(91)90334-c | DOI Listing |
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