Lantang pig (16 sows), which is one of the south China type pig breeds, was crossed with Landrace pig (8 boars) to construct the resource population. According to the pig linkage map of USDA-MARC2.0, 31 microsatellite DNA markers on pig chromosomes 1, 4, 7, and 8 were used to genotype the parents F1 and F2. The distance between adjacent markers was about 10 to 20 cM. The gene frequency, heterozygosity (h), and polymorphism information content (PIC) were calculated. The marker genotype of parents, F1, and F2 were obtained with WAVEO nucleotide fragment analysis system (DHPLC) and ABI 377 DNA sequencer. Twenty-one microsatellite markers on chromosomes 1, 4, and 8 were genotyped with ABI 377. The length of the DNA fragments of 18 alleles on 13 microsatellite markers were beyond the range reported on the web site. The loci of new alleles were 62% of total markers. The heterozygosities of the 31 microsatellite ranged from 0.043 to 0.7855, the heterozygosities of 70% loci were over 0.6, the average heterozygosity was 0.6460. The average polymorphism information content (PIC) of 31 microsatellite markers was 0.5949, the PIC of 77.4% loci were over 0.5. The values of h and PIC suggested that polymorphism information of these markers in the resource population was plentiful. These markers could be used to map quantitative traits loci of important economic traits in this population.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1360/yc-007-0817 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Genetic diversity and population structure analysis of short-day onions using molecular markers in association with resistance to Fusarium basal rot.
Physiol Plant
January 2025
Horticulture Crops Research Department, Hormozgan Agricultural and Natural Resources Research and Education Center, AREEO, Bandar Abbas, Iran.
In this research, we analyzed Random Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeats (ISSR) and Sequence-related amplified polymorphism (SRAP) markers to evaluate the genetic diversity of eighteen different onion genotypes with various resistant levels to FOC. The results showed that the polymorphism means between RAPD primers was 61.11 to 81.
View Article and Find Full Text PDFComprehensive analysis of LMNB2 in pan-cancer and identification of its biological role in sarcoma.
Aging (Albany NY)
July 2024
Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong Province, China.
Backgrounds: Sarcoma (SARC) is a mesenchymal tumor which often responds poorly to systemic therapy. It is therefore important to look for possible biological markers that could tell the prognosis and the progression of SARC.
Methods: A combined evaluation of the Cancer Genome Atlas (TCGA) and genotypic tissue expression (GTEx) portal was used to analyzeLMNB2 expression level in different types of cancer.
APOBEC3A deaminates CTG hairpin loops to promote fragility and instability of expanded CAG/CTG repeats.
Proc Natl Acad Sci U S A
January 2025
Program in Genetics, Molecular, and Cellular Biology, Tufts University Graduate School of Biomedical Sciences, Boston, MA 02111.
CAG/CTG repeats are prone to expansion, causing several inherited human diseases. The initiating sources of DNA damage which lead to inaccurate repair of the repeat tract to cause expansions are not fully understood. Expansion-prone CAG/CTG repeats are actively transcribed and prone to forming stable R-loops with hairpin structures forming on the displaced single-stranded DNA (S-loops).
View Article and Find Full Text PDFGenetic Diversity and Relationships Among Tunisian Wild and Cultivated L. Species.
Plants (Basel)
December 2024
LR99ES12, Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie, Faculté des Sciences de Tunis, Université de Tunis El Manar, Tunis 2092, Tunisia.
Assessing and determining genetic diversity in rose species is a crucial step for conservation efforts, the establishment of a core collection, and the development of new varieties. This study represents the first investigation of genetic diversity among various rose species at different ploidy levels in Tunisia, with the aim of elucidating the genetic structure of the genus. It encompasses both spontaneous and cultivated accessions, featuring local and introduced species recognized for their adaptability, ornamental value, and fragrance.
View Article and Find Full Text PDFGenetic Diversity and Identification of Species Through Microsatellite Analysis.
Plants (Basel)
December 2024
Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes e Alto Douro (UTAD), 5000 Vila Real, Portugal.
The genus contains about 500 species distributed worldwide but only a limited number of species have been studied for genetic diversity using molecular markers. In this study, a genetic analysis was conducted on three Vaccinium species (four cultivars of , four wild populations of , and two cultivars of ), for a total of 95 genotypes, using eight microsatellite (SSR) loci. A total of 57 alleles were detected.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!