Cobalt is toxic for cells, but mechanisms of this toxicity are largely unknown. The biochemical and genetic experiments reported here demonstrate that iron-sulfur proteins are greatly affected in cobalt-treated Escherichia coli cells. Exposure of a wild-type strain to intracellular cobalt results in the inactivation of three selected iron-sulfur enzymes, the tRNA methylthio-transferase, aconitase, and ferrichrome reductase. Consistently, mutant strains lacking the [Fe-S] cluster assembly SUF machinery are hypersensitive to cobalt. Last, expression of iron uptake genes is increased in cells treated with cobalt. In vitro studies demonstrated that cobalt does not react directly with fully assembled [Fe-S] clusters. In contrast, it reacts with labile ones present in scaffold proteins (IscU, SufA) involved in iron-sulfur cluster biosynthesis. We propose a model wherein cobalt competes out iron during synthesis of [Fe-S] clusters in metabolically essential proteins.
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http://dx.doi.org/10.1074/jbc.M702519200 | DOI Listing |
Pol J Vet Sci
December 2024
Department of Animal Nutrition and Husbandry, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, Košice, 04181, Slovakia.
The present study aimed to search for the presence of the plasmid-mediated antimicrobial resistance genes in 106 Escherichia coli (E. coli) isolates from a total of 240 fresh fecal samples collected from 12 private cattle farms in Bingol province of East Turkey from November 2021 to January 2022. In those colistin-resistant E.
View Article and Find Full Text PDFPorcine circovirus type 2 (PCV2) is the major causative agent of postweaning multisystemic wasting syndrome which leads to significant economic losses in the global swine industry. In China, there is a widespread dissemination of PCV2 infection in the pig population. Serological diagnosis of the disease is considered as an effective control measure.
View Article and Find Full Text PDFFront Biosci (Elite Ed)
December 2024
Discipline of Microbiology, School of Life Sciences, College of Agriculture, Engineering and Science, Westville Campus, University of KwaZulu-Natal, 4000 Durban, South Africa.
Background: () is the most prominent bacterial pathogen that causes urinary tract infections (UTIs), and the rate of resistance to most used antibiotics is alarmingly increasing.
Methods: This study assessed the hostel gutters of two Nigerian universities, the University of Nigeria, Nsukka (UNN) and Kogi State University, Anyigba (KSU), for and its antimicrobial resistance genes (). Oxoid Chromogenic UTI agar was used to isolate uropathogenic (UPEC), identified using standard biochemical tests.
J West Afr Coll Surg
July 2024
Department of Surgery, Faculty of Clinical Sciences, Usmanu Danfodiyo University, Usmanu Danfodiyo University Teaching Hospital, Sokoto, Nigeria.
Background: Necrotizing fasciitis (NF) is a severe soft tissue infection typified by swiftly spreading necrosis of the fascia and subcutaneous fat with successive necrosis of the skin which affects all age groups.
Objective: To compare the clinical presentation and treatment outcome of NF between children and adults.
Materials And Methods: A prospective descriptive study of all patients presenting with NF to the (Usmanu Danfodiyo Univrersity Teaching Hospital, Sokoto), from September 2018 to August 2019.
Curr Res Microb Sci
November 2024
Microbiology and Plant Pathology Laboratory, Department of Botany, Jai Narain Vyas University, Jodhpur, Rajasthan, India.
Medicinal plants exhibited great role in drug industries. Herbal medicines and their derivative products are often prepared from crude plant extracts. and both are belonging to Asteraceae family and these plants are ethnomedicinally important due to their utilization as traditional medicine to cure various diseases.
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