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An exchangeable SIM probe for monitoring organellar dynamics of necrosis cells and intracellular water heterogeneity in kidney repair.
Proc Natl Acad Sci U S A
January 2025
Institute of Optical Materials and Chemical Biology, Guangxi Key Laboratory of Electrochemical Energy Materials, School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, Guangxi, People's Republic of China.
Monitoring subcellular organelle dynamics in real time and precisely assessing membrane heterogeneity in living cells are very important for studying fundamental biological mechanisms and gaining a comprehensive understanding of cellular processes. However, there remains a shortage of effective tools for these purposes. Herein, we propose a strategy to develop the exchangeable water-sensing probeAPBD for time-lapse imaging of dynamics in cellular membrane-bound organelle morphology with structured illumination microscopy at the nanoscale.
View Article and Find Full Text PDFMembrane-embedded CdaA is required for efficient synthesis of second messenger cyclic di-AMP.
Commun Biol
December 2024
Department of Biochemistry, Groningen Biomolecular Science and Biotechnology Institute, University of Groningen, Nijenborgh 3, Groningen, The Netherlands.
Cyclic di-adenylate monophosphate (cyclic di-AMP) is an important second messenger in microorganisms. Cyclic di-AMP regulates bacterial cell volume and turgor via control of potassium and compatible solute transport but is also involved in many other processes, including the activation of the metazoan innate immune response to bacterial infections. We compare the activity of full-length membrane-embedded CdaA, the enzyme that synthesizes cyclic di-AMP, with the water-soluble catalytic domain CdaA-DAC.
View Article and Find Full Text PDFWater-membrane partition and the mutant selection window of antimicrobial peptides: insights from liposome studies.
J Colloid Interface Sci
December 2024
Department of Chemical Science and Technologies, Tor Vergata University of Rome, 00133 Rome, Italy. Electronic address:
The mutant selection window (MSW) is a range of antimicrobial concentrations, where some bacteria are killed, while others survive. Within this interval resistance may develop. Antimicrobial peptides (AMPs) are a promising class of antimicrobials that generally act by perturbing the integrity of bacterial membranes.
View Article and Find Full Text PDFSymmetry breaking and mismatch in the torsional mechanism of ATP synthesis by FF-ATP synthase: mathematical number theory proof and its chemical and biological implications.
Theory Biosci
December 2024
Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India.
Can mathematical proofs be employed for the solution of fundamental molecular-level problems in biology? Recently, I mathematically tackled complex mechanistic problems arising during the synthesis of the universal biological currency, adenosine triphosphate (ATP) by the FF-ATP synthase, nature's smallest rotary molecular motor, using graph-theoretical and combinatorial approaches for the membrane-bound F and water-soluble F domains of this fascinating molecule (see Nath in Theory Biosci 141:249‒260, 2022 and Theory Biosci 143:217‒227, 2024). In the third part of this trilogy, I investigate another critical aspect of the molecular mechanism-that of coupling between the F and F domains of the ATP synthase mediated by the central γ-subunit of nanometer diameter. According to Nath's torsional mechanism of energy transduction and ATP synthesis the γ-subunit twists during ATP synthesis and the release of stored torsional energy in the central γ-stalk causes conformational changes in the catalytic sites that lead to ATP synthesis, with 1 ATP molecule synthesized per discrete 120° rotation.
View Article and Find Full Text PDFHigh stability of the radical at the catalytic center of cytochrome c oxidase.
Arch Biochem Biophys
February 2025
Center for Interdisciplinary Biosciences, Technology and Innovation Park, University of P. J. Safarik, Jesenna 5, 041 54, Kosice, Slovak Republic. Electronic address:
In aerobic organisms, cellular respiration is associated with electron transfer through a respiratory system of membrane-bound complexes. This electron flow is terminated by the reduction of dioxygen to water by respiratory oxidases. Cytochrome c oxidase (CcO) is a widely distributed heme-copper-oxygen reductase (HCO) found in all mitochondria and some bacteria.
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