The purpose of this review is to discuss the use of chicken and other model organisms in the study of B-cell development and function as well as to highlight the opportunities afforded by the expanded genome-sequencing efforts. A brief introduction on chicken B-cell biology is followed by discussion of somatic cell reverse genetic approaches using the DT40 cell line. The unique advantages of the DT40 system are emphasized with discussion on B-cell receptor signalling research as well as on DNA repair and mechanisms of immunoglobulin diversification. An attempt is made to compare and contrast the results from chicken with mouse knockouts on the one hand and RNAi with human cell lines on the other. Chicken is also emerging strongly as a platform for gene expression analysis, and avian studies are compared with mammalian studies. Multi-species gene co-expression analysis, which could also be termed phylotranscriptomics, aims to use the evolutionary distance between organisms to its advantage. This approach, still in its infancy, is also reviewed and its applicability to the chicken is discussed.
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