[Bioluminescent imaging monitoring of a anti-angiogenesis therapeutic gene vasostatin in tumor cell PC3].

Objective: To generate a sensitive tool for noninvasive monitoring of a therapeutic gene vasostatin.

Methods: We fused the bioluminescent reporter gene firefly luciferase to the therapeutic transgene vasostatin and ensured that these two proteins would not interrupt each other and kept their own natural character.

Results: We therefore examined clones of PC3 cells stably expressing fusion gene and positive controlfluc with bioluminescence. In vivo imaging of PC3-Fluc subcutaneous tumors showed that the mean tumor bioluminescence increased in animals over several weeks.

Conclusion: Noninvasive monitoring facilitates the detection of gene expression in vivo and in vitro.