[Effects of siRNA on expression of livin and dose- and time-response in human malignant melanoma LiBr cells].

Aim: To investigate the effects of siRNA on expression of livin and dose- and time-response in human malignant melanoma LiBr cells.

Methods: Three chemically synthesized specific siRNAs targeting to livin were transfected to LiBr cells, then the expression of livin was detected both at mRNA level by real-time RT-PCR and at protein level by Western blot, and the effective one was selected for use on observation of dose- and time-response to livin silencing.

Results: One of three designed siRNAs could effectively knock down the livin expression both at mRNA and protein levels in dose- and time- dependent manners, 100 nmol/L of which achieved the highest knockdown effect on mRNA at 48 h and on protein at 72 h after transfection.

Conclusion: Expression of livin in LiBr cells could be efficiently knocked down by siRNA with target site 790-808 of livin mRNA.