A primitive genetic code, composed of a smaller set of amino acids, may have expanded via recursive periods of genetic code ambiguity that were followed by specificity. Here we model a step in this process by showing how genetic code ambiguity could result in an enhanced growth rate in Acinetobacter baylyi.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1951902 | PMC |
| http://dx.doi.org/10.1128/JB.00622-07 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Computational Methods for Lineage Reconstruction.
Methods Mol Biol
January 2025
Centro Nacional de Análisis Genómico, Barcelona, Spain.
The recent development of genetic lineage recorders, designed to register the genealogical history of cells using induced somatic mutations, has opened the possibility of reconstructing complete animal cell lineages. To reconstruct a cell lineage tree from a molecular recorder, it is crucial to use an appropriate reconstruction algorithm. Current approaches include algorithms specifically designed for cell lineage reconstruction and the repurposing of phylogenetic algorithms.
View Article and Find Full Text PDFStarTrack: Mapping Cellular Fates with Inheritable Color Codes.
Methods Mol Biol
January 2025
Instituto Cajal, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
StarTrack is a powerful multicolor genetic tool designed to unravel cellular lineages arising from neural progenitor cells (NPCs). This innovative technique, based on retrospective clonal analysis and built upon the PiggyBac system, creates a unique and inheritable "color code" within NPCs. Through the stochastic integration of 12 distinct plasmids encoding six fluorescent proteins, StarTrack enables precise and comprehensive tracking of cellular fates and progenitor potentials.
View Article and Find Full Text PDFEfficient genome editing in medaka () using a codon-optimized SaCas9 system.
J Zhejiang Univ Sci B
December 2024
Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affairs, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system, belonging to the type II CRISPR/Cas system, is an effective gene-editing tool widely used in different organisms, but the size of Cas9 (SpCas9) is quite large (4.3 kb), which is not convenient for vector delivery. In this study, we used a codon-optimized Cas9 (SaCas9) system to edit the tyrosinase (, oculocutaneous albinism II (), and paired box 6.
View Article and Find Full Text PDFGenetically Recoding Respiratory Syncytial Virus to Visualize Nucleoprotein Dynamics and Virion Assembly.
ACS Infect Dis
January 2025
Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, Missouri 63130, United States.
RNA viruses possess small genomes encoding a limited repertoire of essential and often multifunctional proteins. Although genetically tagging viral proteins provides a powerful tool for dissecting mechanisms of viral replication and infection, it remains a challenge. Here, we leverage genetic code expansion to develop a recoded strain of respiratory syncytial virus (RSV) in which the multifunctional nucleoprotein is site-specifically modified with a noncanonical amino acid.
View Article and Find Full Text PDFHistone mark age of human tissues and cell types.
Sci Adv
January 2025
Department of Computer Science, Brown University, Providence, RI, USA.
Aging is a complex and multifaceted process involving many epigenetic alterations. One key area of interest in aging research is the role of histone modifications, which can dynamically regulate gene expression. Here, we conducted a pan-tissue analysis of the dynamics of seven key histone modifications during human aging.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!