AI Article Synopsis
- A new 32-fold pinhole and photomultiplier detection array has been developed for multifocal multiphoton microscopy to minimize blurring from scattered light in biological samples.
- This method enhances photon statistics for detecting ballistic photons by four times and improves spatial resolution by 21% laterally and 35% axially compared to traditional single-beam techniques.
- The new system has been tested on plant leaves and pollen grains, confirming significant improvements in imaging quality.
Article Abstract
We have developed a new descanned parallel (32-fold) pinhole and photomultiplier detection array for multifocal multiphoton microscopy that effectively reduces the blurring effect originating from scattered fluorescence photons in strongly scattering biological media. With this method, we achieve a fourfold improvement in photon statistics for detecting ballistic photons and an increase in spatial resolution by 21% in the lateral and 35% in the axial direction compared to single-beam non-descanned multiphoton microscopy. The new detection concept has been applied to plant leaves and pollen grains to verify the improvements in imaging quality.
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| http://dx.doi.org/10.1117/1.2736425 | DOI Listing |
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