A simple and selective one-step solid-phase extraction procedure using chemically modified polymer resin (Amberlite XAD-4) with crown ether was investigated for the measurement of urinary catecholamines. After loading the urine samples (adjusted to pH 4) on the synthesized adsorbent cartridge, the column was washed with methanol followed by water and then the adsorbed catecholamines were eluted by 1.0 mL of 6.0 M acetic acid. The effectiveness of sample clean-up method was demonstrated by reversed-phase ion-pair high-performance liquid chromatography with electrochemical detection. Under optimal condition, the recoveries of epinephrine, norepinephrine, and dopamine from spiked urine sample were >86% for all catecholamines. The detection limits (n=5) for epinephrine, norepinephrine, and dopamine were 37, 52, and 46 nmol/L, respectively.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.chroma.2007.06.033 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Identification of 3-methoxytyramine as a specific biomarker for beet-sugar-fed honey: A two year surveillance study in South Korea.
Food Res Int
January 2025
New Hazardous Substances Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Osong, Cheongju, Chungcheongbuk-do 28159, Republic of Korea. Electronic address:
Honey is highly vulnerable to food fraud, and there are growing concerns about product authenticity. The commonly used stable carbon isotope ratios in the Calvin (C3) and Hatch-Slack (C4) photosynthesis cycles in plant feed cannot distinguish between beet-sugar-fed honey and natural honey. However, 3-methoxytyramine (3-MT) can be used as specific biomarker for identifying adulteration of beet-sugar-fed honey.
View Article and Find Full Text PDFCharacterization of key flavor compounds in cinnamon bark oil extracts using principal component analysis.
Food Res Int
January 2025
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China. Electronic address:
Cinnamon is a widely used spice, known for its distinctive flavor and aromatic properties. Due to its lignified structure, the release of flavor components typically requires prolonged stewing (1-2 h). To simulate the release of flavor components during stewing, this study employed corn oil for extraction, avoiding the use of organic solvents.
View Article and Find Full Text PDFUiO-66 with missing cluster defects for high-efficient extraction and enrichment of benzoylurea insecticides.
J Chromatogr A
January 2025
Henan International Joint Laboratory of Medicinal Plants Utilization, College of Chemistry and Molecular Sciences, Henan University, Kaifeng 475004, Henan, China. Electronic address:
The creation of defects in crystalline structures can tune metal-organic frameworks (MOFs) properties, such as improving their adsorptive and catalytic performance with producing more porosity and active sites. In this work, the bimetallic UiO-66 containing Zn and Zr was prepared. And then UiO-66 with missing cluster defects (UiO-66-1/3) were obtained by acid washing to remove the Zn nodes.
View Article and Find Full Text PDFDetermination of clostebol residues in the urine of slaughter animals using liquid chromatography-tandem mass spectrometry.
J Vet Res
December 2024
Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Puławy, Poland.
Introduction: Synthetic anabolic hormones, which may pose a potential risk to human health, should not be used in fattening food-producing animals. Because of the hormonal effects they cause, growth-promoting compounds are banned by legislation in the EU for use in animal husbandry. Consequently, all EU member states are required to conduct monitoring tests on the content and residues of these compounds in prescribed biological matrices to ensure the safety of food consumers.
View Article and Find Full Text PDFFirst Step on the Way to Identify Dermatophytes Using Odour Fingerprints.
Mycopathologia
January 2025
Laboratory of Fungal Genetics and Metabolism, Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic.
The clinical diagnosis of dermatophytosis and identification of dermatophytes face challenges due to reliance on culture-based methods. Rapid, cost-effective detection techniques for volatile organic compounds (VOCs) have been developed for other microorganisms, but their application to dermatophytes is limited. This study explores using VOCs as diagnostic markers for dermatophytes.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!