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Establishing piperacillin-tazobactam susceptibility in ceftriaxone non-susceptible Enterobacterales: comparing disk diffusion, Etest, and VITEK 2 automated minimal inhibitory concentration measurements vs. broth microdilution.
Clin Microbiol Infect
November 2024
Division of Infectious Diseases, Department of Medicine, McGill University Health Centre, Montréal, Québec, Canada; Clinical Practice Assessment Unit, Department of Medicine, McGill University Health Centre, Montréal, Québec, Canada.
Objectives: Post-hoc analyses of the MERINO trial highlight the uncertainty associated with establishing piperacillin-tazobactam (PTZ) susceptibility in extended-spectrum beta-lactamase-producing Enterobacterales. Herein, we compare the concordance of susceptibility for PTZ among the VITEK 2, disc diffusion, and Etest with broth microdilution (BMD) as the reference standard.
Methods: Ninety-four consecutive ceftriaxone non-susceptible Escherichia coli and Klebsiella pneumoniae bloodstream isolates were identified from patients at three hospitals in Montréal, Québec.
Detecting Echinocandin Resistance in C. glabrata Using Commercial Methods: Are CLSI or EUCAST Breakpoints Suitable for Categorical Classification?
Mycoses
November 2024
Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Universidad Complutense de Madrid, Madrid, Spain.
Background: Previous studies correlated Sensititre YeastOne and gradient diffusion plastic strips with standard procedures for the detection of echinocandin-resistant C. glabrata isolates. However, these studies were limited by the low number of resistant isolates studied; the inclusion of sufficient numbers of mutant isolates is essential to test the procedures' capacity to detect resistance.
View Article and Find Full Text PDFCarbapenem non-susceptibility overcalling by BD phoenix NMIC-500 panel.
J Infect Chemother
October 2024
Department of Laboratory Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea. Electronic address:
We aimed to assess the accuracy of BD Phoenix for determining carbapenem susceptibility because we observed a decline in carbapenem susceptibility rate from the biannual cumulative data, after we transitioned to the BD Phoenix form Vitek 2 system. Between October 2021 and May 2022, we collected 82 non-duplicated Enterobacterales showing non-susceptible to at least one of the three carbapenems by BD Phoenix. We performed the broth microdilution (BMD) and disk diffusion (DD) according to the CLSI guideline.
View Article and Find Full Text PDFMolecular Characterization and Sterol Profiles Identify Nonsynonymous Mutations in as a Major Mechanism Conferring Reduced Susceptibility to Amphotericin B in Candida kefyr.
Microbiol Spectr
August 2023
Department of Microbiology, Faculty of Medicine, Kuwait University, Safat, Kuwait.
The molecular basis of reduced susceptibility to amphotericin B (rs-AMB) among any yeasts is poorly defined. Genetic alterations in genes involved in ergosterol biosynthesis and total cell sterols were investigated among clinical Candida kefyr isolates. C.
View Article and Find Full Text PDF[The Importance of Gram-stained Smears in the Diagnosis of Nocardia Infections].
Mikrobiyol Bul
January 2023
Hacettepe University Faculty of Medicine, Department of Medical Microbiology, Ankara, Türkiye.
Nocardia species are low virulence bacteria found in nature. They can be an infectious agent, especially in patients with risk factors such as underlying immunosuppression, chronic lung disease, and malignancy. They can be easily overlooked because they are not seen frequently and has no pathognomonic symptoms.
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