AI Article Synopsis
- The study aimed to evaluate the toxicity of orthodontic adhesives using a three-dimensional model of human oral tissue.
- The adhesives tested were Transbond XT and Excite, with results showing acute toxicity for specific applications, particularly the Excite primer and Transbond XT primer, while the native cell cultures showed no toxic reactions.
- Overall, the research demonstrated that the reconstructed human oral epithelium is an effective model for assessing the toxicity of dental materials, highlighting concerns about certain uncured adhesives.
Article Abstract
Objective: To determine the toxicity of orthodontic adhesives assessed on in vitro three-dimensional reconstructed human oral epithelium (RHOE).
Materials And Methods: Two adhesive primers, Transbond XT (3M, Monrovia, Calif) and Excite (Vivadent, Schaan, Liechtenstein), were tested. After topical exposure, the cell cultures were fixed, cut, and stained for light microscopy (LM) and transmission electron microscopy (TEM). Detection of cytotoxicity by measuring lactate dehydrogenase (LDH) activity was performed. Toxicity was assessed by evaluating the morphological changes with LM and TEM. Copper wires and Triton X-100 served as positive controls, and native cell cultures as negative control.
Results: Morphological evaluation of the native cell cultures revealed no toxic reactions. The LDH assay revealed the following mean values for viability: native cell line (negative control), 1.51; Triton X-100 (positive control), 3.06; Transbond XT polymerized, 1.15; Excite polymerized, 1.11; Transbond XT primer, 2.67; and Excite primer, 0.04. Acute toxicity was observed for Triton X-100 and Transbond XT primer (P < .001). Histological evaluation of the RHOE showed toxicity for both primers and mild changes after topical application of polymerized adhesives. The biocompatibility ranking of the adhesive primers was the same after histological analysis and LDH assay except for Excite noncured.
Conclusions: RHOE proved to be a valuable model for topical exposure. The toxicity of both uncured primers was demonstrated.
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| http://dx.doi.org/10.2319/052706-212.1 | DOI Listing |
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