AI Article Synopsis
- Tsukamurella paurometabola C-924, a nematocide, was cultured in a large 300 l bioreactor and formulated into spray-dried powders using sucrose.
- The powders demonstrated varying survival rates of 12-85% at a drying temperature of 62 degrees Celsius with sucrose concentrations of up to 10%.
- Stability studies indicate that vacuum storage at 4 degrees Celsius is optimal, and a new method for analyzing bacterial cell death dynamics at low temperatures was established, revealing an Arrhenius behavior in cell death kinetics for vacuum-stored powders.
Article Abstract
The nematocidal agent, Tsukamurella paurometabola C-924, was cultured in a 300 l bioreactor. Spray-dried formulations of this microorganism were prepared using sucrose. At an outlet temperature 62 degrees C, survival rates between 12 and 85% were reached with sucrose up to 10% (w/w). The stability study of the powders showed that the best storage condition was at 4 degrees C under vacuum. A new method for the calculation of cell death order for bacteria stored at low temperatures was developed. Powders stored under vacuum showed an Arrhenius behavior in relation to cell death kinetics.
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| http://dx.doi.org/10.1007/s10529-007-9436-0 | DOI Listing |
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