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[Growth inhibition and multidrug resistance-reversing effect of tanshinone I A on human breast cancer cell with estrogen receptor negative]. | LitMetric

[Growth inhibition and multidrug resistance-reversing effect of tanshinone I A on human breast cancer cell with estrogen receptor negative].

Sichuan Da Xue Xue Bao Yi Xue Ban

Experimental Oncology Laboratory, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.

Published: June 2007

AI Article Synopsis

  • The study explores the effects of tanshinone I A on human ER negative breast cancer cells, focusing on its ability to inhibit growth and reverse multidrug resistance (MDR).
  • Experiments showed that tanshinone I A treatment significantly reduced cell proliferation and colony formation while altering cell cycle distribution and increasing specific gene expressions related to cancer response.
  • The results indicate that tanshinone I A's effectiveness is linked to its cytotoxic properties, including the inhibition of DNA synthesis and modulation of apoptosis, cell cycle arrest, and changes in gene expression.

Article Abstract

Objective: To investigate the growth inhibition and multidrug resistance (MDR) reversing effect of tanshinone I A on human breast cancer cells with estrogen receptor (ER) negative, and to elucidate its mechanism of activity.

Methods: Human ER negative breast cancer cells (MDA-MB-231) were tested in vitro for cytotoxicity and colony formation inhibition. Brdu incorporation and cell cycle distribution were also checked through flow cytometry (FCM). With RT-PCR, the expressions of ADP-ribosyltransferase CNAD+; poly (ADP-ribose) polymerase)-like 1 (ADPRTL1), cytochrome P450 subfamily I (CYP1A1) and breast cancer resistance protein (BCRP/ABCG2) mRNA were detected for testing the response to tanshinone 1 A treatment.

Results: After tanshinone I A treatment, the proliferation, colony formation and Brdu labeling indices of cancer cells decreased (P<0. 05). By FCM analysis, the increase of subG, and G0/G1 phase cell populations and decrease of S and G2/M phase cells were observed (P
Conclusion: The findings in these studies suggest that tanshinone I A exhibits the potent cytotoxicity and MDR reversing potential to human ER negative breast cancer cells. The mechanism for such effects may be associated with the inhibition of DNA synthesis, induction of apoptosis, cell cycle arrest and up-regulation of ADPRTL1, CYP1A1, and down-regulation of BCRP/ABCG2 expression in cancer cells.

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