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Metabolic Engineering of BL21(DE3) for 2'-Fucosyllactose Synthesis in a Higher Productivity.
ACS Synth Biol
January 2025
College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China.
2'-Fucosyllactose (2'-FL) is the most abundant human milk oligosaccharides (HMOs). 2'-FL exhibits great benefits for infant health, such as preventing infantile diarrhea and promoting the growth of intestinal probiotics. The microbial cell factory technique has shown promise for the massive production of 2'-FL.
View Article and Find Full Text PDFDifferent Types of Co-Infection by Contagious Ecthyma Virus, Enteropathogenic , , Ecto- and Endo-Parasites in Four Young Alpine Ibex ().
Animals (Basel)
December 2024
Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Regione Amerique 7G, 11020 Quart, AO, Italy.
The research aimed to investigate the perinatal pathology of Alpine ibex () through the study of four young subjects (at the age of 3 to 4 months) found dead in Valle d'Aosta, a region of northwestern Italy. The carcasses were submitted to necropsy followed by an examination of ecto- and endoparasites (ECP and ENP); samples from the gross lesions (in summary, cutaneous papilloma and crusts, ocular discharge, lobular haemorrhagic areas in the lungs, catarrhal-haemorrhagic enterocolitis) were analysed by bacteriological, histopathological, and biomolecular methods to define the etiological agent. The subjects, with various co-infection patterns, were affected by contagious ecthyma virus (ORFV) (agent of a highly diffusive pustular dermatitis transmissible to small ruminants and humans), Enteropathogenic (EPEC) (major etiological agent of infantile diarrhoea especially in developing countries), (MC) (cause of an ocular infection common to goats and sheep), various ECP (ticks and keds) and ENP (lung and intestinal nematodes, and coccidia).
View Article and Find Full Text PDFHuman milkoligosaccharides: Role in lactation and infant formulas.
Arch Argent Pediatr
December 2024
Instituto de Lactología Industrial (INLAIN, CONICET-UNL), Facultad de Ingeniería Química, Universidad Nacional del Litoral, Santa Fe, Argentina.
Human milk oligosaccharides (HMO) are the specific and selective growth substrate for bifidobacteria, preventing pathogen adhesion, modulating the immune system, and impacting neurodevelopment. Human milk is the best food for the neonate; infant formulas enriched with HMOs are indicated when human milk is not possible or sufficient. HMOs developed and added to available infant formulas are 2'-FL (2'-fucosyl lactose), 3-FL (3-fucosyl lactose), 3'-SL (3'-sialyl lactose), 6'-SL (6'-sialyl lactose), LNT (lacto-N-tetraose), and 3'-GL (3'-galactosyl lactose), the latter being produced in situ by microbial fermentation.
View Article and Find Full Text PDFDiagnosis of perianal pyramidal protrusion in infants.
Dermatol Online J
October 2024
Department of Dermatology, University of Florida College of Medicine, Gainesville, Florida.
Infantile perianal pyramidal protrusion is characterized by a light pink to skin-colored soft tissue protrusion that is often midline and anterior [A1]to the anus. It most commonly occurs in young females and is relatively asymptomatic. Although biopsies are not routinely done, histopathology is relatively nonspecific and can appear similar to an acrochordon.
View Article and Find Full Text PDFLong-term Gastrointestinal Sequelae in Children who Underwent Pyloromyotomy for Infantile Hypertrophic Pyloric Stenosis.
J Pediatr Surg
November 2024
Department of Pediatric Surgery, Emma Children's Hospital, Amsterdam UMC, University of Amsterdam and Vrije Universiteit Amsterdam, the Netherlands; Amsterdam Gastroenterology Endocrinology Metabolism, Meibergdreef 9, Amsterdam, the Netherlands; Amsterdam UMC, Amsterdam Reproduction and Development Research Institute, University of Amsterdam, Meibergdreef 9, Amsterdam, the Netherlands.
Article Synopsis
- The study aimed to assess the long-term gastrointestinal effects of pyloromyotomy in children who had undergone this surgery for infantile hypertrophic pyloric stenosis (IHPS) from 2007 to 2017.
- A questionnaire, including the Pediatric Quality of Life Inventory™ Gastrointestinal symptoms module, was completed by 199 participants, revealing that their GI symptoms scores were similar to those of healthy peers.
- The results suggest that long-term GI issues after pyloromyotomy are rare, indicating no significant difference in health outcomes between these children and healthy controls, which can help inform parents and lessen the need for extensive follow-up care.
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