Although dietary modification is a common treatment strategy used to manage dysphagic patients who aspirate thin liquids, there are no standard definitions for thickened liquid preparation. This lack of standardization leads to variability in practice and points to the need for a simple tool for clinicians to assess thickened liquid consistency. The current study analyzed the utility of the Line Spread Test (LST) in this regard. Twenty-six liquids (10 powder-thickened "nectar" juices, 10 powder-thickened "honey" juices, and 6 barium mixtures) were assessed using both a viscometer for objective measurement of viscosity and the LST. Whereas the LST was able to separate the juices into nectar and honey categories, it was not able to separate barium mixtures into these categories nor compare barium to juices. Furthermore, the LST was not predictive of viscosity. Thus, the results of the current study suggest that the LST may be useful in the broad categorization of fluids into therapeutically significant groupings but that it cannot be used more specifically to measure fluid viscosity. Further studies of this and other tools are necessary to identify inexpensive practical tools for quantification of thickened liquid consistency.
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http://dx.doi.org/10.1007/s00455-007-9086-3 | DOI Listing |
Acta Dermatovenerol Croat
November 2024
Prof. Branka Marinović, MD, PhD, Department of Dermatology and Venereology, University Hospital Center Zagreb, School of Medicine University of Zagreb, Kišpatićeva 12, 10000 Zagreb.
Psychological stress may affect skin barrier homeostasis and slow down its recovery. Therefore, we aimed to examine the association between psychological stress levels and natural moisturizing factor (NMF) levels in the stratum corneum (SC). NMF levels were determined in the SC of 25 third-year and 25 sixth-year medical students in two periods (stressful and non-stressful) using the tape stripping method, high-performance liquid chromatography, and spectrophotometry.
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January 2025
School of Advanced Technology, Xi'an Jiaotong-Liverpool University, Suzhou, 215000, China.
Alzheimer's disease (AD) is the leading cause of dementia worldwide, and the development of early screening methods can address its significant health and social consequences. In this paper, we present a rotary-valve assisted paper-based immunoassay device (RAPID) for early screening of AD, featuring a highly integrated on-chip rotary micro-valve that enables fully automated and efficient detection of the AD biomarker (amyloid beta 42, Aβ42) in artificial plasma. The microfluidic paper-based analytical device (μPAD) of the RAPID pre-stores the required assay reagents on a μPAD and automatically controls the liquid flow through a single valve.
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January 2025
Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA, USA.
Histological techniques to study muscle are crucial for assessing skeletal muscle health. To preserve tissue morphology, samples are usually fixed in formaldehyde or cryopreserved immediately after excision from the body. Freezing samples in liquid nitrogen, using isopentane as a mediator for efficient cooling, preserves the tissue in its natural state.
View Article and Find Full Text PDFBio Protoc
January 2025
Department of Biochemistry, Microbiology and Biotechnology, Kenyatta University, Nairobi, Kenya.
Agrobacterium-mediated gene transformation method is a vital molecular biology technique employed to develop transgenic plants. Plants are genetically engineered to develop disease-free varieties, knock out unsettling traits for crop improvement, or incorporate an antigenic protein to make the plant a green factory for edible vaccines. The method's robustness was validated through successful transformations, demonstrating its effectiveness as a standard approach for researchers working in plant biotechnology.
View Article and Find Full Text PDFJ Diabetes Metab Disord
June 2025
Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Objectives: Non-alcoholic fatty Liver Disease (NAFLD) poses a growing global health concern, yet its complex aetiology remains incompletely understood. Emerging evidence implicates the gut microbiome and choline metabolism in NAFLD pathogenesis. This study aims to elucidate the association of choline-consuming bacteria in gut microbiome with choline level.
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