AI Article Synopsis
- - cDNA for Insulin-like growth factor binding protein 3 (IGFBP3) was cloned and used to create a prokaryotic expression vector, pET-DsBA-IGFBP3, which was then transformed into E. coli BL21 (DE3) plysS to produce a fusion protein called D-IGFBP3.
- - D-IGFBP3 was successfully expressed in a soluble form and purified to over 95% purity using His affinity chromatography.
- - The effectiveness of D-IGFBP3 was confirmed through Western-blot analysis, and the cell assays demonstrated that it can inhibit the growth of MCF-7 cancer cells and bind to IGF-I in vitro.*
Article Abstract
cDNA for Insulin-like growth factor binding protein 3 was cloned and constructed a prokaryotic expression vector--pET-DsBA-IGFBP3. The construct was transformed into E. coli BL21 (DE3)plysS. The induced fusion protein (D-IGFBP3) was expressed successfully in soluble form. We obtained D-IGFBP3 the purify of which is over 95% after purification by His affinity chromatography. The product was identified by Western-blot. The cell assay showed that the obtained fusion protein can inhibit the growth of MCF-7 and bind with IGF-I in vitro.
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