Despite all the advances in nonlinear microscopy, all existing instruments are constrained to obtain images of one focal plane at a time. In this Letter we demonstrate a two-photon absorption fluorescence scanning microscope capable of imaging two focal planes simultaneously. This is accomplished by temporally demultiplexing the signal coming from two focal volumes at different sample depths. The scheme can be extended to three or more focal planes.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1364/ol.32.001731 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Fabrication of Artificial Compound Eyes with Biplanar Focal Planes on a Curved Surface.
ACS Appl Mater Interfaces
January 2025
Dalian University of Technology State Key Laboratory of High-Performance Precision Manufacturing, Key Laboratory for Micro/Nano Technology and System of Liaoning Province, School of Mechanical Engineering, Dalian 116024, China.
Inspired by ancient trilobites, novel curved microlens arrays (CMLAs) were designed. Direct, fast, and low-cost CMLAs with two focal planes were fabricated using ultraprecision machining technology and hot embossing technology. We designed four pairs of artificial compound eyes (ACEs) composed of large and small lenses with four different curvatures to achieve focusing and imaging on two focal planes.
View Article and Find Full Text PDFIn this Letter, we present a novel, to the best of our knowledge, approach for recovering objects directly from the Fraunhofer diffraction integral, where the diffraction field of an object is approximated by the Fourier transform of this object augmented by an additional phase factor. This phase factor at the observation plane is universal for the diffraction fields generated by objects located at the same plane and illuminated by the same monochromatic plane wave. It can be first extracted from dividing the Fraunhofer diffraction field by the Fourier transform of an object reference.
View Article and Find Full Text PDFWastewater-based analysis of antimicrobial resistance at UK airports: Evaluating the potential opportunities and challenges.
Environ Int
January 2025
School of Environmental & Natural Sciences, Bangor University, Bangor LL57 2UW, UK; Verily Life Sciences LLC., South San Francisco, CA 94080, United States.
With 40 million annual passenger flights, airports are key hubs for microbial communities from diverse geographic origins to converge, mix, and distribute. Wastewater derived from airports and aircraft represent both a potential route for the global dispersion of antimicrobial resistant (AMR) organisms and an under-utilised resource for strengthening global AMR surveillance. This study investigates the abundance and diversity of antimicrobial resistance genes (ARGs) in wastewater samples collected from airport terminals (n = 132), aircraft (n = 25), and a connected wastewater treatment plant (n = 11) at three international airports in the UK (London Heathrow, Edinburgh and Bristol).
View Article and Find Full Text PDFTuning the spin state of the iron center by FePc/Mg(OH) heterojunction boosting oxygen reduction performance.
J Colloid Interface Sci
January 2025
National-Local Joint Engineering Laboratory for Energy Conservation in Chemical Process Integration and Resources Utilization, School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130 PR China. Electronic address:
Iron phthalocyanine (FePc) is a promising non-noble metal catalyst for oxygen reduction reaction (ORR). While, with the plane-symmetric FeN site, the ORR activity of FePc is generally low due to its low ability to adsorb and activate O. Herein, we anchor FePc on Mg(OH)/N-doped carbon nanosheets building the ternary plate-like catalyst FePc/Mg(OH)/NC.
View Article and Find Full Text PDF3D fluorescence staining and confocal imaging of low amount of intestinal organoids (enteroids): Protocol accessible to all.
PLoS One
January 2025
Laboratory of Hepato-Gastroenterology, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, Belgium.
The emerging field of 3D organ modeling encounters several imaging issues in particular related to antigen retrieval and sample loss during staining processes. Due to their compact shape, several antibodies fail to penetrate intact organoids or spheroids. Histology of organoids can be approached by paraffin inclusion and sectioning at 5 μm as performed for biopsies.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!