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Optimized methods for scRNA-seq and snRNA-seq of skeletal muscle stored in nucleic acid stabilizing preservative.
Commun Biol
January 2025
Center for Research on Genomics and Global Health, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Single cell studies have transformed our understanding of cellular heterogeneity in disease but the need for fresh starting material can be an obstacle, especially in the context of international multicenter studies and archived tissue. We developed a protocol to obtain high-quality cells and nuclei from dissected human skeletal muscle archived in the preservative Allprotect® Tissue Reagent. After fluorescent imaging microscopy confirmed intact nuclei, we performed four protocol variations that compared sequencing metrics between cells and nuclei enriched by either filtering or flow cytometry sorting.
View Article and Find Full Text PDFHierarchically aligned heterogeneous core-sheath hydrogels.
Nat Commun
January 2025
Institute of Innovative Materials, Department of Chemistry, College of Science, Southern University of Science and Technology, Shenzhen, China.
Natural materials with highly oriented heterogeneous structures are often lightweight but strong, stiff but tough and durable. Such an integration of diverse incompatible mechanical properties is highly desired for man-made materials, especially weak hydrogels which are lack of high-precision structural design. Herein, we demonstrate the fabrication of hierarchically aligned heterogeneous hydrogels consisting of a compactly crosslinked sheath and an aligned porous core with alignments of nanofibrils at multi-scales by a sequential self-assembly assisted salting out method.
View Article and Find Full Text PDFConstruction of in situ modulated controlled growth of MOF-on-mof impedimetric assembly for the practical minimal level assessment of anti-mullerian hormone.
Biosens Bioelectron
December 2024
Department of Biological Science and Technology, Institute of Molecular Medicine and Bioengineering, Center for Intelligent Drug Systems and Smart Bio-devices (IDS(2)B), Yang Ming Chiao Tung University, Hsinchu 300, Taiwan; Department of Biomedical Science and Environmental Biology, School of Dentistry, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan. Electronic address:
Anti-mullerian hormone (AMH) detection receives much attention since it is used as an ideal biomarker for quantitative assessment of ovarian reserve. The present study proposed a first report on the use of MOF-on-MOF as an electrochemical sensor for recognizing AMH in buffer and serum media. The MOF-on-MOF, MIL-88 B@UiO66NH was synthesized by the internal extended growth method (IEGM) involving MIL-88 B on UiO66NH by in situ method for the first time.
View Article and Find Full Text PDFToward a Science of Emergence: Definitions, Prototypes, Principles and Applications.
Nonlinear Dynamics Psychol Life Sci
January 2025
University of Massachusetts, Boston, MA.
Emergence as a phenomenon is embedded and expressed in the natural world, and in social systems. Introduced nearly 150 years ago in a philosophical context, it has since been applied in nearly every natural and social science. However all of these uses are not congruent, as the range of emergences in this Special Issue reflect as well; this has limited the accumulation of knowledge about emergence, as well as its development as a discipline.
View Article and Find Full Text PDFSecupyritines A‒C, Three Natural Propellane Securinega Alkaloids: Structure Elucidation and Total Synthesis Based on Biogenetic Building Blocks.
Angew Chem Int Ed Engl
January 2025
Jinan University, State Key Laboratory of Bioactive Molecules and Druggability Assessment, CHINA.
Secupyritines A‒C are unique polycyclic Securinega alkaloids isolated from medicinal plant Flueggea suffruticosa. They feature a distinctive 6/6/6/5/6 fused pentacyclic ring system with a highly strained 2-oxa-6-aza[4.4.
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