Objectives: Cervical intraepithelial neoplasias (CIN) associated with high-risk (HR) human papillomavirus infection, in addition to HR-HPV typing need other viral marker testing to distinguish a subset of lesions with clinical relevant infections. This study has evaluated the significance of viral markers, such as viral load, physical status and E2/E6 ratio, to stratify HPV16 infected women at a single point in time for grade of cervical lesions.
Methods: One hundred sixty-six cytological specimens were selected from women with low (n=72) and high (n=94) grade squamous intraepithelial lesions (SIL), and positive to HPV16. All the 72 LSIL were CINI, 83 of the 94 HSIL were CINII/III and 11 SCC (Squamous Cervical Carcinoma). Cytological specimens were analysed by two different SYBR Green Real-time PCR assays (RT-PCR). Specific primers for both E2 and E6 viral genes and GAPDH cellular gene were designed to determine viral load, physical status and E2/E6 ratio.
Results: The viral load was significantly higher in HSIL than in LSIL. In CINI episomal DNA was prevalent (72.2%), mixed forms (episomal and integrated) were 27.8%, suggestive of an early integration of viral DNA into cellular genome, no pure integrated forms were detected. However in CINII/III mixed DNA forms were prevalent (73.5%). In SCC pure integrated DNA was prevalent (81.8%) in absence of episomal forms. E2/E6 ratio decreased significantly from CINI to CINII/III and SCC with a linear trend. The logistic regression analysis showed that viral load higher than 1.38x10(6) genome copies per 300 ng of total DNA associated with E2/E6 ratio lower than 0.90 was highly significant in differentiating CINII/III versus CINI, while the only E2/E6 value lower than 0.17 was significant in differentiating SCC from CINI.
Conclusions: Viral load higher than 1.38x10(6) genome copies per 300 ng of total DNA and E2/E6 ratio values allow HPV16 infected women with high grade cervical intraepithelial lesions to be recognized.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ygyno.2007.05.004 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
HPV Detection in Breast Tumors and Associated Risk Factors in Northeastern Brazil.
Cells
June 2024
Laboratory of Molecular Studies and Experimental Therapy, Department of Genetics, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235. Cidade Universitária Recife, Pernambuco, Recife 50670901, PE, Brazil.
Breast cancer risk factors include lifestyle, genetic-hormonal influences, and viral infections. Human papillomavirus (HPV), known primarily as the etiological agent of cervical cancer, also appears active in breast carcinogenesis, as evidenced in our study of 56 patients from northeastern Brazil. We assessed the clinical and sociodemographic characteristics, correlating them with various breast cancer tumor types.
View Article and Find Full Text PDFEvaluation of human papillomavirus type 16 viral load and genome physical status in Iranian women with cervical disease.
Mol Biol Rep
March 2024
Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
Article Synopsis
- - This study focused on analyzing the presence and condition of the HPV-16 virus in various cervical lesions, including non-cancerous, precancerous, and cancerous tissues.
- - Researchers used quantitative real-time PCR to measure the viral loads of two HPV-16 genes (E2 and E6) in 132 cervical samples, finding significant differences in these loads across the lesion types.
- - The results indicated that the E2 gene viral load is a strong biomarker for differentiating lesion types, with E2 and E6 showing high specificity and sensitivity in identifying cancerous samples.
HPV vaccination status and effectiveness in Korean women with HPV16/18 infection (2010-2021): a retrospective study.
J Gynecol Oncol
September 2024
Division of Clinical Research, Center for Emerging Virus Research, National Institute of Infectious Diseases, Korea National Institute of Health, Cheongju, Korea.
Objective: To evaluate human papillomavirus (HPV) vaccine effectiveness in a cohort of Korean women infected with HPV.
Methods: From 2010 to 2021, Korean women aged 20-60 years who diagnosed HPV-positive atypical squamous cells of undetermined significance or low-grade squamous intraepithelial lesion were recruited from 6 hospitals. HPV vaccine effectiveness was estimated by observing the differences in pathological and clinical information and experimental results-prevalence, viral load (VL), physical state (PS), and HPV16/18 infection duration-between the vaccinated and unvaccinated groups.
Human Papillomavirus 16 DNA Methylation Patterns and Investigation of Integration Status in Head and Neck Cancer Cases.
Int J Mol Sci
September 2023
Department of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Article Synopsis
- Persistent high-risk HPV infection is crucial to the development of cervical cancer and increasingly linked to head and neck squamous cell carcinoma (HNSCC), with HPV-positive cases having distinct characteristics compared to HPV-negative ones.
- This study investigates the epigenetic changes in the HPV16 gene and untranslated region (UTR) using pyrosequencing in HPV16-positive HNSCC biopsies, focusing on DNA methylation patterns.
- Results show significant methylation at specific sites associated with tumor histological grade, while HPV16 DNA physical status did not relate to methylation or tumor features, highlighting complex interactions between the virus and host in HNSCC patients in Greece.
hnRNP K induces HPV16 oncogene expression and promotes cervical cancerization.
J Cancer Res Clin Oncol
August 2023
Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan, China.
Purpose: This study aims to explore the expression of hnRNP K in cervical carcinogenesis and to investigate the regulatory role of hnRNP K on HPV16 oncogene expression as well as biological changes in cervical cancer cells.
Methods: In total 1042 subjects, including 573 with the normal cervix and 469 with different grades of cervical lesions were enrolled in this study to explore the association between hnRNP K and HPV16 oncogene expression in cervical carcinogenesis. Additionally, the Gene Omnibus (GEO) database was used to analyze hnRNP K mRNA expression in cervical cancerization.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!