Problem: To determine the value of leukemia inhibitory factor (LIF) assessment for predicting the reproductive outcome.
Method Of Study: Two phase study. Phase I: assessment of LIF in uterine flushing. Phase II: 1,5 years after examining the last patient, a questionnaire was sent to all participants of the phase I. Phase I: Uterine flushing and endometrial samples were collected during implantation window from infertile patients with stage I/II endometriosis (n = 14), patients with idiopathic infertility (n = 27), luteal phase deficiency (n = 13), and fertile control (n = 21). LIF was assessed in uterine flushings in all patients by ELISA. In endometrium, semiquantitative RT-PCR was performed for LIF mRNA expression. Phase II: questionnaire has been sent to all infertile women taking part in the first phase of the experiment, regarding their reproductive outcome.
Results: 65.4% patients who had returned the questionnaire did get pregnant. LIF concentration at a cut-off point of 2.31 pg/ml had a 95.7% sensitivity and 81.8% specificity in predicting the reproductive outcome.
Conclusion: This prospective study for the first time in literature indicates that the LIF assessment can be used as a predictor of reproductive success.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1600-0897.2007.00492.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The reproductive tract microbiome in women with polycystic ovary syndrome and across different menstrual cycle phases.
Hum Reprod
January 2025
Institute of Genomics, Estonian Genome Centre, University of Tartu, Tartu, Estonia.
Study Question: Do polycystic ovary syndrome (PCOS), menstrual cycle phases, and ovulatory status affect reproductive tract (RT) microbiome profiles?
Summary Answer: We identified microbial features associated with menstrual cycle phases in the upper and lower RT microbiome, but only two specific differences in the upper RT according to PCOS status.
What Is Known Already: The vaginal and uterine microbiome profiles vary throughout the menstrual cycle. Studies have reported alterations in the vaginal microbiome among women diagnosed with PCOS.
Role of resistin in the porcine uterus: effects on endometrial steroidogenesis.
Reprod Fertil Dev
January 2025
Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
Context The adipose tissue produces adipokines - hormones essential to many biological functions, including reproduction. Aims We hypothesised that resistin, one of the adipokines, is present in the blood plasma, uterine luminal flushings (ULF) and uterus of pigs during the oestrous cycle and early pregnancy, and that resistin influences uterine steroidogenesis. Methods This study aimed to determine the expression of resistin in the porcine endometrium and myometrium during the cycle and pregnancy by quantitative real-time polymerase chain reaction and western blot (WB).
View Article and Find Full Text PDFEfficacy of uterine flushing with human chorionic gonadotropin (hCG) on pregnancy rates in primary unexplained infertility: a randomized controlled trial.
Eur J Med Res
December 2024
Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Background: There are limited and controversial findings concerning ovulation induction using intrauterine and intramuscular human chorionic gonadotropin (hCG) injection compared to intramuscular hCG alone. The study aimed to examine the impact of intrauterine hCG injection, which is used to induce ovulation, on the efficacy of the intrauterine insemination (IUI) technique in patients with unexplained infertility.
Methods: A randomized controlled clinical trial was conducted involving 80 subjects with unexplained primary infertility at the infertility clinic of Al-Zahra Hospital in northwest Iran.
Bushen Huoxue decoction improves the reproduction of endometriosis-associated infertility by regulating Homeobox A10 and αlpha(v)beta(3) integrin expression.
J Tradit Chin Med
December 2024
Department of Obstetrics and Gynecology, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China.
Objective: To investigate the mechanism of Bushen Huoxue decoction (, BSHXD) to treat endometriosis-induced infertility.
Medhods: The main compounds of BSHXD were determined by high performance liquid chromatography-mass spectrometry (HPLC-MS/MS). The effect of BSHXD on Homeobox A10 (HOXA10) and alpha(v)beta(3) (αvβ3) integrin expression of Ishikawa cells, mouse model, and endometriosis-associated infertility women was evaluated by using Western blot analysis, immunohistochemistry and Real-Time quantitative polymerase chain reaction (RT-qPCR).
Presence of embryos increases uterine blood flow and affects endometrial gene expression in Holstein cows on day seven post-insemination.
Reprod Biol
November 2024
Clinic of Reproductive Medicine, Vetsuisse Faculty Zurich, Winterthurerstrasse 260, 8057 Zurich, Switzerland.
Superovulation treatment leads to characteristic changes in uterine perfusion during the first three weeks of pregnancy in cattle, but the origin of these changes is unknown. We investigated embryo-induced effects on uterine blood flow volume (uBFV) and pulsatility index (uPI), as well as on steroid hormone concentrations during the first seven days after conception, together with endometrial gene expression at the time of embryo flushing, in Holstein Friesian cows. Animals (n = 8) underwent (a) artificial insemination (AI), (b) superovulation followed by artificial insemination (SOV-AI), and (c) superovulation followed by sham AI (SOV-control) in a Latin square design.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!