The resazurin assay utilising microtitre-plate, described by Drummond and Waigh in 2000, has been modified to achieve more accuracy in the determination of the minimum inhibitory concentration (MIC) values of natural products, including crude extracts, chromatographic fractions or purified compounds against various bacterial strains. This modified resazurin method is simple, sensitive, rapid, robust and reliable, and could be used successfully to assess antibacterial properties of natural products.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1895922 | PMC |
http://dx.doi.org/10.1016/j.ymeth.2007.01.006 | DOI Listing |
Int J Biol Macromol
January 2025
College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Provincial Engineering Technology Research Center of Prefabricated Seafood Processing and Quality Control, Zhanjiang 524088, China. Electronic address:
Degumming, a process of removing gummy substances surrounding fiber, plays a crucial role in preparing plant fibers. This study clearly clarified that the multiple degumming enzymes by Bacillus subtilis XW-18 acted as a decisive factor for driving bio-degumming process of raw pineapple leaves. Firstly, PCR analysis verified that B.
View Article and Find Full Text PDFSci Total Environ
January 2025
China National Environmental Monitoring Centre, Beijing 100012, China.
The riverine dissolved organic matter (DOM) pool constitutes the largest and most dynamic organic carbon reservoir within inland aquatic systems. Human activities significantly alter the distribution of organic matter (OM) in rivers, thereby affecting the availability of DOM. However, the impact of total suspended solids (TSS) on DOM under anthropogenic influence remains insufficiently elucidated.
View Article and Find Full Text PDFSmall
January 2025
National Energy Metal Resources and New Materials Key Laboratory, Engineering Research Center of the Ministry of Education for Advanced Battery Materials, Hunan Provincial Key Laboratory of Nonferrous Value-Added Metallurgy, School of Metallurgy and Environment, Central South University, Changsha, 410083, P. R. China.
Electrochemical CO reduction (CORR) in membrane electrode assembly (MEA) represents a viable strategy for converting CO into value-added multi-carbon (C) compounds. Therefore, the microstructure of the catalyst layer (CL) affects local gas transport, charge conduction, and proton supply at three-phase interfaces, which is significantly determined by the solvent environment. However, the microenvironment of the CLs and the mechanism of the solvent effect on C selectivity remains elusive.
View Article and Find Full Text PDFOrg Biomol Chem
January 2025
State Key Laboratory and Institute of Elemento-Organic Chemistry, Frontiers Science Center for New Organic Matter, College of Chemistry, Nankai University, Tianjin 300071, China.
A 6-step gram-scale synthesis of the fungal polyketide (±)-gregatin A is described. The synthetic route features an intermolecular 1,3-dipolar cycloaddition, a Mo-mediated disconnection of the isoxazole skeleton, and an acid-mediated deprotection/enamine hydrolysis and hemiketalization cascade.
View Article and Find Full Text PDFChem Biodivers
January 2025
Zhejiang University, Polytechnic Institute, 866 Yuhangtang Road, Hangzhou, CHINA.
Filamentous fungi are of great interest due to their powerful metabolic capabilities and potentials to produce abundant various secondary metabolites as natural products (NPs), some of which have been developed into pharmaceuticals. Furthermore, high-throughput genome sequencing has revealed tremendous cryptic NPs underexplored. Based on the development of in silico genome mining, various techniques have been introduced to rationally modify filamentous fungi,awakening the silent biosynthetic gene clusters (BGCs) and visualizing the NPs originally cryptic.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!