The purification of human antibodies in an aqueous two-phase system (ATPS) composed of polyethylene glycol (PEG) 6000 and phosphate was optimised by surface response methodology. A central composite design was used to evaluate the influence of phosphate, PEG and NaCl concentration and of the pH on the purity and extraction yield of IgG from a simulated serum medium. The conditions that maximise the partition of IgG into the upper phase were determined to be high concentrations of NaCl and PEG, low concentrations of phosphate and low pH values. An ATPS composed of 12% PEG, 10% phosphate, 15% NaCl at pH 6 was further used to purify human monoclonal antibodies from a Chinese Hamster Ovary (CHO) concentrated cell culture supernatant with a recovery yield of 88% in the upper PEG-rich phase and a purification factor of 4.3. This ATPS was also successfully used to purify antibodies from a hybridoma cell culture supernatant with a recovery yield of 90% and a purification factor of 4.1.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jbiotec.2007.04.002 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Memory Effect of Double Oxides Compared to Simple Ion Exchange for Controlled Fluoride Ion Capture and Release.
Materials (Basel)
January 2025
Faculty of Technology and Metallurgy, University of Belgrade, 11000 Belgrade, Serbia.
Article Synopsis
- A magnesium-aluminum layered double hydroxide (LDH) was created using a coprecipitation technique from a nitrate solution and transformed into a layered double oxide (LDO) after being heated to 450 °C.
- During rehydration in a fluoride solution, the LDH's original structure was restored and fluoride ions were absorbed to maintain balance, a finding confirmed by energy-dispersive X-ray spectroscopy (EDS).
- The study demonstrated that using ethanol during the rehydration process significantly increased fluoride incorporation, and the fluoride release pattern from the material revealed a rapid initial release followed by a slower, prolonged release.
Process optimization, structural characterization, and biological activity of Siraitia grosvenorii polysaccharides by ultrasonic aqueous two-phase extraction.
Int J Biol Macromol
January 2025
Guangxi Key Laboratory of Fruits and Vegetables Storage-processing Technology, Guangxi Academy of Agricultural Sciences, Nanning, China; School of Chemistry and Chemical Engineering, Guangxi University, Nanning, China. Electronic address:
In this research, ultrasound-assisted aqueous two-phase extraction (UA-ATPE) was utilized to isolate polysaccharides from Siraitia grosvenorii (SGPs). Based on the results of model optimization experiments, the extraction parameters were determined. The total extraction rate of SGPs was 25.
View Article and Find Full Text PDFProcessable and controllable all-aqueous gels based on high internal phase water-in-water emulsions.
Mater Horiz
January 2025
Department of Chemical and Biomolecular Engineering, Seoul National University of Science and Technology (SeoulTech), Seoul 01811, Republic of Korea.
Aqueous two-phase systems (ATPSs) have primarily been developed in the form of emulsions to enhance their utilization in green and biocompatible applications. However, numerous challenges have arisen in forming stable and processable water-in-water (W/W) emulsion systems, as well as in fine-tuning the interconnectivity of their internal structure, which can significantly impact their performance. To effectively address these challenges, we elucidate, for the first time, the root cause of the poor stability of W/W emulsions.
View Article and Find Full Text PDFApplication of aqueous two-phase extraction for separation and purification of various adeno-associated viruses.
Biotechnol Lett
January 2025
Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Zhejiang Key Laboratory of Smart Biomaterials, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310058, China.
Objective: Adeno-associated viruses (AAVs) are widely used as gene therapy vectors due to their safety, stability, and long-term expression characteristics. The objective of this work is to develop an aqueous two-phase system (ATPS) as a universal platform for the separation and purification of AAVs.
Results: This study utilized polyethylene glycol (PEG)/salt ATPSs to separate and purify various AAV serotypes, including AAV5, AAV8, and AAV9, which focusing on serotype-specific performance and partial empty capsid removal.
Green Pre-Treatment Strategy Using Ionic Liquid-Based Aqueous Two-Phase Systems for Pesticide Determination in Strawberry Samples.
Foods
December 2024
Department of Physical Chemistry, VINČA Institute of Nuclear Sciences-National Institute of the Republic of Serbia, University of Belgrade, Mike Petrovića Alasa 12-14, 11351 Belgrade, Serbia.
Pesticides used in agriculture can contaminate foods like fruits and vegetables, posing health risks to consumers and highlighting the need for effective residue monitoring. This study explores aqueous two-phase systems (ATPSs) comprising phosphonium or ammonium ionic liquids (ILs) combined with ammonium sulfate as an alternative pre-treatment method for extracting and concentrating the pesticides clomazone, pyraclostrobin, and deltamethrin from strawberry samples. Liquid-liquid equilibrium measurements for each ATPS were conducted, followed by extraction experiments to determine the most efficient systems for pesticide extraction.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!