The S-adenosylhomocysteine hydrolase from the apicomplexan Cryptosporidium parvum (CpSAHH) has been characterized. CpSAHH is a single-copy, intronless gene of 1479 bp encoding a protein of 493 amino acids with a molecular mass of 55.6 kDa. Reverse transcriptase-polymerase chain reaction analysis confirmed that CpSAHH is expressed both in intracellular stages (in C. parvum-infected HCT-8 cells 24 h after infection) and in sporozoites. CpSAHH was expressed in Escherichia coli TB1 cells as a fusion with maltose-binding protein. The recombinant fusion was cleaved by Factor Xa and the enzymatic activity of both the fusion protein and the purified separated CpSAHH was measured. The enzymatic activity of CpSAHH was inhibited by d-eritadenine, S-DHPA and Ara-A.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1574-6968.2007.00795.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Dysmorphic Findings in SAHH Deficiency with a Novel Variant in the Gene.
Mol Syndromol
December 2024
Department of Medical Genetics, University of Health Sciences, Van Training and Research Hospital, Van, Turkey.
Introduction: S-adenosylhomocysteine hydrolase (SAHH) is one of the enzymes involved in converting methionine to homocysteine with transmethylation processes. Methyltransfer reactions are impaired in SAHH deficiency. SAHH deficiency is multisystemic and antenatal onset disorder.
View Article and Find Full Text PDFS-adenosylmethionine and S-adenosyl-L-homocysteine metabolism is involved in the sperm motility and in vitro fertility rate in mouse.
Biochem Biophys Res Commun
December 2024
Department of Cellular Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan; Neutron Therapy Research Center, Okayama University, Okayama, Japan. Electronic address:
Increased fragmentation of sperm DNA has been implicated in male infertility. Folate deficiency results in impaired methionine synthesis, depletion of S-adenosylmethionine (SAM) levels, an increase in S-adenosyl-l-homocysteine (SAH) levels, and increased DNA fragmentation. Disruption of the dynamic balance between SAM and SAH may also contribute, although the details of this process are not yet fully understood.
View Article and Find Full Text PDFGenome-wide DNA methylation regulated by AHCY through SAM / SAH axis promotes psoriasis pathogenesis.
J Dermatol Sci
December 2024
Institute of Dermatology and Hospital for Skin Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing, China. Electronic address:
Article Synopsis
- Psoriasis is a widespread chronic skin condition, and the role of S-adenosine homocysteine hydrolase (AHCY) in its development and its effects on DNA methylation had not been thoroughly examined.
- This study explored how AHCY expression in psoriatic lesions relates to disease severity and its effects on keratinocyte behavior and DNA methylation through knockdown experiments.
- The results show that AHCY is elevated in psoriasis, contributes to disease severity, and alters DNA methylation patterns, suggesting that targeting AHCY could be a potential therapeutic approach for treating psoriasis.
Asymptomatic pediatric presentation of S-adenosylhomocysteine hydrolase deficiency.
JIMD Rep
November 2024
Article Synopsis
- S-adenosylhomocysteine hydrolase deficiency is an autosomal recessive metabolic disorder disrupting the methionine cycle, which can range from severe symptoms to asymptomatic cases.
- Two clinically asymptomatic siblings from Pakistan were diagnosed with mild chronic liver failure and other mild symptoms, with one sibling showing improvements after dietary changes.
- The research highlights a specific genetic variant common in South Asia and suggests this milder form of the disease may be underdiagnosed, emphasizing the need for better therapeutic management to prevent future health complications.
In memory of an exquisite medicinal chemist, Prof. Morris Robins.
Nucleosides Nucleotides Nucleic Acids
November 2024
Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, Laboratory of Virology and Chemotherapy, KU Leuven, B-3000Leuven, Belgium.
Among the most prominent realizations of Morris J. Robins in the antiviral nucleoside chemistry are the synthesis of 8-substituted (methyl-, amino-, bromo-, iodo) derivatives of acyclovir, xylotubercidin as an inhibitor of herpes simplex virus (HSV) infections, the anti-HIV activity of the 2',3'-dideoxyriboside of 2,6-diaminopurine (ddDAPR) and the 3'-azido- and 3'-fluoro derivatives thereof (AzddDAPR and FddDAPR, respectively), the potentiating effect of ribavirin on the anti-HIV activity of 2',3'-dideoxyinosine (ddI) and ddDAPR, S-adenosylhomocysteine hydrolase (SAH) inhibitors principally active against vaccinia virus (VV) and vesicular stomatitis virus (VSV), and furo[2,3-d]pyrimidinone derivatives active against varicella-zoster virus (VZV).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!