AI Article Synopsis
- E-cadherin plays a vital role in cell adhesion during embryonic development, influencing how cells organize and migrate.
- Culturing F9 and P19 embryonal carcinoma cells on an E-cadherin matrix leads to scattered distributions and changes in their cytoskeletal structures, in contrast to more organized colonies observed on other matrices.
- Immature embryonic cells show unique migratory behaviors influenced by E-cadherin, while differentiated cells (like mammary, kidney, and liver cells) do not exhibit this scattering, indicating different regulatory mechanisms based on cell differentiation.
Article Abstract
Rearrangement of cell-cell adhesion is a critical event in embryonic development and tissue formation. We investigated the regulatory function of E-cadherin, a key adhesion protein, in the developmental process by using E-cadherin/IgG Fc fusion protein as an adhesion matrix in cell culture. F9 embryonal carcinoma cells usually form colonies when cultured on gelatin or fibronectin matrices. However, F9 cells cultured on the E-cadherin/IgG Fc fusion protein matrix formed a scattered distribution, with a different cytoskeletal organization and E-cadherin-rich protrusions that were regulated by Rac1 activity. The same scattering activity was observed in P19 embryonal carcinoma cells. In contrast, three types of differentiated cells, NMuMG mammary gland cells, MDCK kidney epithelial cells, and mouse primary isolated hepatocytes, did not show the scattering activity observed in F9 and P19 cells. These results suggest that migratory behavior on an E-cadherin-immobilized surface is only observed in embryonic cells, and that the regulatory mechanisms underlying E-cadherin-mediated cell adhesion vary with the state of differentiation.
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| http://dx.doi.org/10.1002/jcb.21406 | DOI Listing |
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