[Oxidative stress of human derived fetal hepatocytes induced by product of chlorinated drinking water (MX)].

Objective: To study the oxidative stress induced by 3-chloro-4-( dichloromethyl)-5-hydroxy-2 [5H]-furanone (MX) (a product of chlorinated drinking water) in human derived fetal hepatocytes (L-02) in vitro.

Methods: L-02 cells were treated at the doses of 10, 30, 100 and 300 micromol/L of MX for 24h. Malondialdehyde (MDA), reduced glutathione (GSH) and 8-hydroxydeoxyguanosine (8-OHdG), the representative of antioxidative molecules and the marker of DNA oxidative damage respectively, were detected in L-02 cell treated by MX. Dimethyl sulfoxide (DMSO) was used as solvent control.

Results: (1) The content of MDA was significantly increased in L-02 cells etreated by MX at the doses of 30, 100, 300 micromol/L in comparison with solvent control. (2) The level of GSH was significantly decreased and the level of 8-OHdG was significantly increased in L-02 cells treated by MX at the concentration of 100, 300 micromol/L in comparison with solvent control. (3) There was a striking positive association between MDA content and 8-OHdG level (r = 0.767, P < 0.01) and a negative association between GSH level and 8-OHdG level (r = 0.761, P < 0.01) in L-02 cells treated by MX at the doses from 0 to 300 micromol/L.

Conclusion: MX could induce oxidative stress in L-02 cells including increases of lipid peroxidation and DNA oxidative damage, and weakened effect of antioxidation. DNA oxidative damage in L-02 cells might be associated with lipid peroxidation and the weakening of antioxidation induced by MX.