AI Article Synopsis
- The study investigated the characteristics of the EGCG-Cu(II) complex when combined with DNA, specifically focusing on fluorescence, absorption, and resonance light scattering (RLS) spectra.
- In the fluorescence spectra, the intensity increased with DNA concentration, suggesting EGCG-Cu(II) can be used as a DNA detection probe.
- The absorption spectra displayed hyperchromicity, and RLS also showed enhancement, attributed to intercalation and electrostatic interactions between the complex and DNA.
- The effects of acidity and ionic strength on fluorescence intensity were also analyzed.
Article Abstract
The fluorescence spectra, absorption spectra and resonance light scattering (RLS) spectra of EGCG-Cu((II) complex with nucleic acid were studied. Compared with the spectrum of EGCG-Cu(II), the spectrum of EGCG-Cu(II)-DNA system showed the following features: (1) in the fluorescence spectra, the peak did not shift, but the fluorescence intensity was enhanced and was proportional to the concentration of DNA. Under suitable conditions, EGCG-Cu(II) promised to be a probe for detecting DNA; (2) Absorption spectra of EGCG-Cu(II)-DNA system showed hyperchromicity; and (3) RLS of EGCG-Cu(II)-DNA system was enhanced also. A mechanism was suggested that the combination of nucleic acid and EGCG-Cu(II) complex was caused by intercalation and electrostatic force. An intercalation interaction between EGCG-Cu(II) and DNA enhanced the fluorescence intensity. Hyperchromicity of absorption spectra and RLS occurred because of electrostatic force. The effects of acid and ionic strength on fluorescence intensity were also discussed.
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