AI Article Synopsis
- New monomethine cyanine dyes were created using quinoline and benzothiazole and analyzed with various techniques like NMR and spectroscopy.
- These dyes, which include amino and acetamido groups, were synthesized through the reaction of p-toluenesulfonate heterocyclic salts, resulting in iodide, bromide, and tosylate forms.
- When tested as DNA stains in electrophoretic gels, the acetamido group and bromide counteranion showed better sensitivity compared to amine groups and other counteranions, similar to ethidium bromide detection at low DNA concentrations (1-3 ng).
Article Abstract
Some new monomethine cyanine dyes derived from quinoline and benzothiazole have been prepared and characterized by (1)H and (13)C NMR, FTIR, FABHRMS, and visible spectroscopy. The dyes containing amino and acetamido groups were conveniently synthesized by the condensation of two p-toluenesulfonate heterocyclic quaternary salts and were obtained in the forms of iodide, bromide, and tosylate counteranions. These dyes were compared to ethidium bromide as stains for DNA in electrophoretic gels. The overall results obtained for the sensitivity of these dyes suggest the suitability of acetamido moiety over the amine one and bromide as the counteranion when compared with iodide and tosylate, with a similar capacity of DNA detection in relation to the ethidium bromide stain over the concentration range of 1-3ng.
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| http://dx.doi.org/10.1016/j.bmc.2007.05.043 | DOI Listing |
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