AI Article Synopsis
- The study examined the production of surfactin by Bacillus subtilis MZ-7 using different types of media (defined, semi-defined, and complex), while maintaining standardized fermentation conditions.
- Surfactin production was confirmed through various analytical methods, with brain heart infusion media yielding the highest production of 280 mg/L, while Pharmamedia produced 220 mg/L, which could be increased to 300 mg/L with specific supplements.
- Cottonseed-derived medium showed potential as a cost-effective substrate for large-scale production of surfactin, indicating its usefulness in medical and biotechnological applications.
Article Abstract
Background: Commercial medium (Pharmamedia) was investigated for the production of surfactin by Bacillus subtilis MZ-7. Different media (defined, semi-defined, and complex media) were compared for the production of surfactin after fixing the least influential variables in standardized fermentation conditions. Carbohydrate and nitrogen supplements were also tried to improve production in Pharmamedia.
Results: Surfactin production was confirmed using PCR along with other analytical techniques and monitored by RP-HPLC and MALDI-TOF-MS. We found that optimized and brain heart infusion media were best for production of surfactin (280 mg/L) and a relatively comparable production with Pharmamedia (220 mg/L), however, supplementing Pharmamedia with Fe+ (4.0 mM) and sucrose (2 g/L) leads to a maximum production of about (300 mg/L).
Conclusion: Cottonseed-derived medium proved to be a suitable substrate for the production of bioactive substances including surfactin, a useful compound in both medical and biotechnological fields. The medium provided not only higher product accumulations but at considerably lower cost with potential for large scale industrial applications.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1894814 | PMC |
| http://dx.doi.org/10.1186/1475-2859-6-17 | DOI Listing |
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