In vitro shoot regeneration is a developmental process that multiple genes and their interactions are involved in. Initiation of somatic cells stimulated by cytokinin, division of the initiated somatic cells and succeeding shoot meristem development are three key steps in the process. Therefore, exploration of gene expression and their interactions involved in these steps will shed light on our understanding of the molecular mechanism for regulation of plant in vitro shoot regeneration. Here we reviewed the research results of molecular regulation process involved in these steps.
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http://dx.doi.org/10.1360/yc-007-0528 | DOI Listing |
World J Microbiol Biotechnol
January 2025
The Key Laboratory for Extreme-Environmental Microbiology, College of Plant Protection, Shenyang Agricultural University, Shenyang, 110866, China.
The endophytic fungus Serendipita indica (Si) could suppress Phoma arachidicola (Pa) and control peanut web blotch disease. The study evaluated its growth-promoting and disease-resistant effects in two peanut cultivars, Luhua11 and Baisha1016. In vitro experiments and microscopy analysis demonstrated that S.
View Article and Find Full Text PDFPlants (Basel)
December 2024
State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Roses are one of the most important flowers applied to landscape, cut flowers, fragrance and food industries widely. As an effective method for plant reproduction, the regeneration via somatic embryos is the most promising method for breed improvement and genetic transformation of woody plants. However, lower somatic embryogenesis (SE) induction rates and genotypic constraints impede progress in genetic transformation in rose.
View Article and Find Full Text PDFPlants (Basel)
December 2024
Department of Biological and Environmental Sciences and Technologies (Di.S.Te.B.A.), University of Salento, Campus Ecotekne, 73100 Lecce, Italy.
Erben & Raimondo is a rare and endangered taxon, endemic to a limited area on Mount Pizzuta in northwestern Sicily, Italy. Its population is significantly threatened by anthropogenic activities, including fires, overgrazing, and habitat alterations. Temporary immersion systems (TISs) have proven effective for large-scale propagation in various protected species, offering potential for ex situ conservation and population reinforcement of .
View Article and Find Full Text PDFMolecules
December 2024
Department of Biology and Pharmaceutical Botany, Medical University of Lodz, 90-151 Lodz, Poland.
Plants of the genus, known for their rich phytochemical profiles, are used in traditional Chinese, Korean, Japanese, and Indian medicine to treat various ailments, including inflammation, hypertension, diabetes, hyperlipidemia, and cancer. Due to the limited natural availability of these plants, there is a growing interest in utilizing in vitro culture techniques to produce their bioactive compounds sustainably. In this study, the effects are compared of Murashige and Skoog (MS), Woody Plant medium (WP), Gamborg B5 (B5), and Schenk and Hildebrandt (SH) basal media on growth, biomass accumulation, and polyphenolic compound production in shoot cultures of and .
View Article and Find Full Text PDFSci Rep
January 2025
Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, 56212, Republic of Korea.
De novo shoot regeneration, characterized by the emergence of adventitious shoots from excised or damaged tissues or organs in vitro, is regulated by the complex interplay between genetic and epigenetic regulatory mechanisms. However, the specific effect of histone deacetylation on shoot regeneration remains poorly understood. This study investigated the effects of trichostatin A (TSA), a histone deacetylase inhibitor, on shoot regeneration in callus derived from root explants.
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