A simulation-based study to predict the impact of biofilm growth on displacement distributions for flow of water through a supporting packed bed is presented. The lattice Boltzmann method and a directed random walk algorithm are used, and are applied to the system with and without biofilm being present. The aim of this simulation study is to model the anomalous transport dynamics induced by biofilm, as reported in the literature, and thus to study the impact of observation time, delta, on the shape of the displacement distributions (propagators). We believe that this is the first demonstration of a propagator simulation for flow through a complex porous structure modulated by biofilm growth. The propagator distributions undergo a transition from a pre-asymptotic to a Gaussian-shaped distribution with increasing delta. The propagators were simulated for a wide range of delta going up to 500 seconds. This transition occurs with and without biofilm, but is very significantly delayed when biofilm is present due to the consequential development of essentially stagnant regions. The transition can be classified into three stages: a diffusion-dominated stage, a "twin-peak" stage and an advection-dominated stage.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.2166/wst.2007.268 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Piezoelectric biosensor with dissipation monitoring enables the analysis of bacterial lytic agent activity.
Sci Rep
January 2025
Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic.
Antibiotic-resistant strains of Staphylococcus aureus pose a significant threat in healthcare, demanding urgent therapeutic solutions. Combining bacteriophages with conventional antibiotics, an innovative approach termed phage-antibiotic synergy, presents a promising treatment avenue. However, to enable new treatment strategies, there is a pressing need for methods to assess their efficacy reliably and rapidly.
View Article and Find Full Text PDFEffector genes of type III secretion system and biofilm formation in virulent isolates carrying and genes in hospital environment.
J Med Microbiol
January 2025
Laboratory of Molecular Microbiology (Micromol), Institute of Biomedical Sciences, Universidade Federal de Uberlndia, Uberlndia, Minas Gerais, Brazil.
In critically ill patients, the occurrence of multidrug-resistant infection is a significant concern, given its ability to acquire multidrug-resistant, form biofilms and secrete toxic effectors. In Brazil, limited data are available regarding the prevalence of dissemination, and the impact of the type III secretion system (T3SS) on toxin production and biofilm formation in clinical isolates of . This study investigates the dissemination of virulent harbouring the and genes, the presence of T3SS genes and their biofilm-forming capability.
View Article and Find Full Text PDFBacterial strains that are genetically engineered to constitutively produce fluorescent proteins have aided our study of bacterial physiology, biofilm formation, and interspecies interactions. Here, we report on the construction and utilization of new strains that produce the blue fluorescent protein mTagBFP2, the green fluorescent protein sfGFP, and the red fluorescent protein mScarlet-I3 in species , and . Gene fragments, developed to contain the constitutive promoter P , the fluorescent gene of interest as well as , providing resistance to the antibiotic spectinomycin, were inserted into selected open reading frames on the chromosome that were both transcriptionally silent and whose loss caused no measurable changes in fitness.
View Article and Find Full Text PDFKeystone engineers profoundly influence microbial communities by altering their shared environment, often by modifying key resources. Here, we show that in an antibiotic-treated microbial community, bacterial spread is controlled by keystone engineering affecting dispersal- an effect hidden in well-mixed environments. Focusing on two pathogens, non-motile Klebsiella pneumoniae and motile Pseudomonas aeruginosa, we found that both tolerate a β-lactam antibiotic, with Pseudomonas being more resilient and dominating in well-mixed cultures.
View Article and Find Full Text PDFmodel of bacterial biofilm mineralization in complex humid environments: a proof of concept study.
Front Bioeng Biotechnol
January 2025
Department of Biomaterials, Max Planck Institute of Colloids and Interfaces, Potsdam, Germany.
Background: Bacteria in physiological environments can generate mineralizing biofilms, which are associated with diseases like periodontitis or kidney stones. Modelling complex environments presents a challenge for the study of mineralization in biofilms. Here, we developed an experimental setup which could be applied to study the fundamental principles behind biofilm mineralization on rigid substrates, using a model organism and in a tailored bioreactor that mimics a humid environment.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!