[Combination use of rh-bFGF and Bio-Oss collagen to enhance periodontal regeneration: an experimental study in dogs].

Purpose: To evaluate the effectiveness of combined use of rh-bFGF and Bio-Oss collagen compounds on regeneration of the canine periodontal tissues.

Methods: Four adult hybrid canine were selected and bone defect of 5mmx5mmx5mm in size was created in the mesio and distal alveolar crest where the premolars were lost in both sides of the maxilla and mandible. Three methods were used in 3 groups,in the experimental group 1, rh-bFGF and Bio-Oss collagen compounds were implanted,Bio-Gide were overlaid; in the experimental group 2,Bio-Oss collagen compounds were implanted, Bio-Gide were overlaid;in the control group only Bio-Gide were overlaid. The therapeutic results were evaluated by clinical and imaging examination as well as histological index. SAS 6.2 software package was used for paired t test.

Results: 8 weeks post operation, there were various amounts of newly formed alveolar bone,cement and connective tissues in each 3-wall intrabony defect. There was significant difference in alveolar osteogenesis between the control group(1.2mm+/-0.1mm) and the 2 experimental groups (3.7mm+/-0.3mm, 2.3mm+/-0.2mm).The difference was also significant between the two experimental groups (P<0.01). There was no significant difference in newly formed cement and connective tissues among the 3 groups (P>0.05).

Conclusions: Application of rh-bFGF/ Bio-Oss collagen compounds benefits to the regeneration of the alveolar bone and avoid the side effects of root resorption ,bone malunion which often occur after conventional periodontal regeneration therapy.Addition of rh-bFGF/ Bio-Oss collagen compounds was useless to cement genesis and periodontal ligament formation.