Anal Chem
Cargill Global Food Technology Group, Cargill Incorporated, P.O. Box 5702, Minneapolis, Minnesota 55440-5702, USA.
Published: July 2007
A method has been developed for rapid quantification of organic acids using ultraperformance liquid chromatography/electrospray-tandem mass spectrometry (UPLC/ESI-MS-MS) to monitor the metabolism of 10 organic acids during microbial fermentation. Because comprehensive chromatographic separation is not required, analysis time is less than traditional ion chromatography assays, with complete organic acid analyses by UPLC/ESI-MS-MS being achieved in less than 3 min. Quantification is accomplished using nine isotopically labeled organic acids as internal standards. Intrasample precisions for organic acid measurements in fermentation supernatants using this method average 8.9% (RSD). Calibration curves are linear over the range of 0.06-100 microg/mL, and detection limits are estimated at 0.06-1 microg/mL. This method has the potential to demonstrate correlation of organic acid consumption and production by microorganisms with observed growth profiles, novel media formulations, and cellular growth events. Data visualization software has been used to profile organic acid levels during fermentation and correlate these profiles to nutrient supplementation protocols employed during microbial production. The potential use of this capability in computational modeling and simulation of microbial metabolism to accelerate the bioprocess development cycle is recognized.
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http://dx.doi.org/10.1021/ac0624243 | DOI Listing |
Animal
December 2024
Department of Animal Science, Faculty of Veterinary Sciences, Universidad de Concepción, Campus Chillán, Chillán 3812120, Chile. Electronic address:
Climate change and food safety standards have intensified research into plant-based compounds as alternatives to dietary supplements in animal feed. These compounds can reduce enteric methane (CH) emissions and the formation of ruminal ammonia. This study investigated the effects of radiata pine bark extract (PBE) supplementation on CH production, ruminal fermentation parameters, and nutrient disappearance using the rumen simulation technique in diets with different forage-to-concentrate (F:C) ratios.
View Article and Find Full Text PDFThe carbon dioxide (CO) capture and utilization strategy has emerged as an innovative and multifaceted approach to counteract carbon emissions. In this study, a highly porous muffin polyhedral barium (Ba) ̵ organic framework (BaTATB; HTATB = 4,4',4″--triazine-2,4,6-triyl-tribenzoic acid) was synthesized solvothermally. The three-dimensional honeycomb pore architectures were densely populated with Lewis acidic Ba(II) metal sites and basic nitrogen-rich triazines.
View Article and Find Full Text PDFChemSusChem
December 2024
Department of Chemistry, Chungnam National University, Daejeon, 34134, Republic of Korea.
Catalyst design plays a critical role in ensuring sustainable and effective energy conversion. Electrocatalytic materials need to be able to control active sites and introduce defects in both acidic and alkaline electrolytes. Furthermore, producing efficient catalysts with a distinct surface structure advances our comprehension of the mechanism.
View Article and Find Full Text PDFOrganometallics
January 2025
Department of Chemistry, University of Bath, Claverton Down, Bath BA2 7AY, U.K.
The group 1 alumanyls, [{SiN}AlM] (M = K, Rb, Cs; SiN = {CHSiMeNDipp}), display a variable kinetic facility (K < Rb < Cs) toward oxidative addition of the acidic C-H bond of terminal alkynes to provide the corresponding alkali metal hydrido(alkynyl)aluminate derivatives. Theoretical analysis of the formation of these compounds through density functional theory (DFT) calculations implies that the experimentally observed changes in reaction rate are a consequence of the variable stability of the [{SiN}AlM] dimers, the integrity of which reflects the ability of M to maintain the polyhapto group 1-arene interactions necessary for dimer propagation. These observations highlight that such "on-dimer" reactivity takes place sequentially and also that the ability of each constituent Al(I) center to effect the activation of the organic substrate is kinetically differentiated.
View Article and Find Full Text PDFRapid Commun Mass Spectrom
April 2025
Solar System Exploration Division, NASA Goddard Space Center, Greenbelt, Maryland, USA.
Rationale: Extraterrestrial amines and ammonia are critical ingredients for the formation of astrobiologically important compounds such as amino acids and nucleobases. However, conventional methods for analyzing the composition and isotopic ratios of volatile amines suffer from lengthy derivatization and purification procedures, high sample mass consumption, and chromatographic interferences from derivatization reagents and non-target compounds.
Methods: Here we demonstrate a highly efficient method to analyze the composition and compound specific isotopic ratios of C to C amines as well as ammonia based on solid phase micro-extraction (SPME) on-fiber derivatization.
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