Adipose-derived stromal cells (ASCs) possess multiple differentiation potentials and may serve as a cell source, if effectively modulated, for regenerative medicine and tissue engineering. Due to estrogen's function in tissue and organ development through regulating cell proliferation and differentiation, we hypothesized that an estrogen supplement may effectively enhance the multiple differentiation potentials of human ASCs. 17-Beta estradiol (E2) was investigated for modulating in vitro osteogenic and adipogenic differentiation in human ASCs isolated from a healthy female donor. After ASCs' exposure to osteogenic and adipogenic differentiation medium supplemented with different concentrations of E2, osteogenic markers (alkaline phosphatase activity, extracellular matrix, calcium deposition, and osteocalcin expression) and adipogenic parameters (lipid accumulation and differentiated cell population) significantly improved. Estrogen's enhancement is dose dependent and linked to differing alpha and beta estrogen receptors. Our data preliminarily demonstrate that estrogen can modulate the differentiation, and potentially improve the efficiency of ASCs in stem cell-based tissue engineering and regeneration. However, further study is needed to verify the regulatory functions of estrogen on ASC differentiations of donors with different ages and genders.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1089/ten.2006.0317 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Dual-target nanotherapy for vascular endothelium and bone mesenchymal stem cells halt steroid-induced osteonecrosis of the femoral head progression.
J Control Release
January 2025
Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. Electronic address:
Steroid-induced osteonecrosis of the femoral head (SANFH) is a common hip joint disease that imposes a heavy economic burden on society. Patients continue to experience bone necrosis even after discontinuing glucocorticoid therapy, and the specific mechanisms require further investigation. The results of this study demonstrate that exosomes secreted by damaged vascular endothelial cells in SANFH lesions may be a crucial factor leading to abnormal adipogenic differentiation of bone marrow stromal cells (BMSCs).
View Article and Find Full Text PDFALG5 downregulation inhibits osteogenesis and promotes adipogenesis by regulating the N-glycosylation of SLC6A9 in osteoporosis.
Cell Mol Life Sci
January 2025
Department of Orthopedics, The Eighth Affiliated Hospital, Sun Yat-sen University, No. 3025, Shennan Middle Road, Futian District, Shenzhen, 518033, Guangdong, China.
Osteoporosis is characterized by decreased bone mass and accumulation of adipocytes in the bone marrow. The mechanism underlying the imbalance between osteoblastogenesis and adipogenesis in bone marrow mesenchymal stem cells (BMSCs) remains unclear. We found that ALG5 was significantly downregulated in BMSCs from osteoporotic specimens.
View Article and Find Full Text PDF[Generation and Evaluation of Human Umbilical Cord Derived Mesenchymal Stem Cells with Antioxidant Capacity].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
December 2024
Life Science School of Hebei University, Baoding 071000, Hebei Province, China.
Article Synopsis
- The study aimed to prepare antioxidant-capable mesenchymal stem cells (AO-MSC) from human umbilical cords and assess their biological properties.
- The AO-MSC were isolated using a method that involved allowing tissue debris to adhere to the culture surface, while control cells were obtained through traditional collagenase digestion.
- Results demonstrated that AO-MSC displayed higher levels of antioxidant substances and good self-renewal ability, but had a weaker capacity for adipogenic and osteogenic differentiation compared to conventional MSC.
Extracellular fluid viscosity regulates human mesenchymal stem cell lineage and function.
Sci Adv
January 2025
Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
Article Synopsis
- Human mesenchymal stem cells (hMSCs) react to mechanical stimuli like stiffness and fluid viscosity, which impacts their behavior.
- In environments with high fluid viscosity, hMSCs favor an osteogenic (bone-forming) phenotype over an adipogenic (fat-forming) one by altering their actin structure and enhancing cellular activities.
- This research highlights fluid viscosity as an important factor that not only influences hMSC differentiation but also encourages a more immunosuppressive M2 macrophage phenotype.
Preclinical Assessment of Safety and Efficacy of Deferoxamine (DFO)-pretreated Feline Adipose Mesenchymal Stem Cells.
In Vivo
December 2024
Department of Veterinary Medicine, Yanbian University, Yanji, P.R. China;
Background/aim: This study aimed to investigate the safety and efficacy of deferoxamine (DFO) pretreated feline adipose tissue derived mesenchymal stem cells (fATMSCs) for the treatment of inflammatory disorders.
Materials And Methods: fATMSCs were isolated from feline adipose tissue and characterized using flow cytometry for surface marker expression and differentiation assays for adipogenic, osteogenic, and chondrogenic lineages. Different concentrations of DFO were used to evaluate its impact on fATMSC activity.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!