AI Article Synopsis
- Positive PCR controls for HPV diagnostics are hard to obtain due to the varying prevalence of high-risk HPV types in different regions.
- Overlapping oligonucleotides were created specifically for HPV types 18, 31, 45, and 58 to improve testing accuracy.
- Synthetic HPV genes were generated using a 2-step assembly PCR method to enhance the diagnosis of type-specific HPV infections.
Article Abstract
Obtaining positive polymerase chain reaction (PCR) controls for human papillomavirus (HPV) diagnostic tests has been difficult because of prevalence variation in different geographic regions of each high-risk viral type. Overlapping oligonucleotides were designed for HPV-18, HPV-31, HPV-45, and HPV-58 type-specific (TS) sequences. Synthetic HPV viral genes were constructed by 2-step assembly PCR for accurately diagnosing TS HPV infection.
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| http://dx.doi.org/10.1016/j.diagmicrobio.2007.04.003 | DOI Listing |
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