AI Article Synopsis
- The study investigates how the Rhodium (II) acetate compound (Rh1) interacts with plasmid pUC19 DNA at different concentrations, confirming binding after a 24-hour incubation at 37°C through agarose gel electrophoresis.
- Results showed that pUC19 DNA migrated more slowly in the gel, indicating a conformational change due to Rh1 binding, especially at higher molar ratios.
- Atomic force microscopy revealed that Rh1 causes the DNA to unwind, and the Rh1-DNA complexes are stable, forming covalent bonds that act as interstrand adducts.
Article Abstract
Binding of Rhodium (II) acetate [Rh(2)(O(2)CCH(3))(4)] (Rh1) compound with plasmid pUC19 DNA has been studied using different molar ratio of Rh1. After incubation for 24hr at 37 degrees C, binding of the Rh1 to pUC19 DNA was confirmed by agarose gel electrophoresis. The electrophoretic results indicated the slower migration speed for the linearized pUC19 DNA. Conformation change of the DNA after Rh1 binding was also indicated at higher molar ratio of Rh1. The atomic force microscopy images showed that the Rh1 induced the conformation change to unwind pUC19 DNA. The Rh1-DNA complexes are observed very stable due to covalent bond. This study clearly demonstrates that [Rh(2)(O(2)CCH(3))(4)] reacts with pUC19 DNA and covalently binds to be stable Rh1-pUC19 DNA as interstrand adducts.
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| http://dx.doi.org/10.1080/07391102.2007.10507144 | DOI Listing |
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