Myocardial biopsies are an increasingly important tool to unravel the molecular mechanisms of cardiac disease. We evaluate a novel minimally invasive trans-thoracic approach for left-ventricular (LV) intra-mural biopsies, which enables repetitive individual sampling in adult dogs. Forty three generally anaesthesised dogs were studied during sinus rhythm (SR, control) and multiple times after the induction of volume overload hypertrophy (complete atrioventricular block [AVB]). Through a small cutaneous incision, an automatic biopsy needle was advanced into the apicolateral LV, guided by fluoroscopy. Electrocardiography (ECG), LV pressure and echocardiography served to monitor the procedure. One hundred eighty-eight intra-mural LV biopsies were obtained in 82 serial experiments (usually SR, 1, 2 and 6 weeks AVB) with a maximum of 8 repeated biopsies. All biopsies ( approximately 10 mm(3)) were suitable for simultaneous application of different cell-biological (light and electron microscopy, immunohistochemistry) and molecular techniques (PCR, Western blotting). In chronic experiments, repeated biopsy sampling did not influence haemodynamics, mechanics, electrocardiographic parameters or myocardial remodelling during SR or AVB. The rate of significant complications was as low as 4% of experiments. Minimally invasive sampling of LV needle biopsies enables serial assessment of myocardial remodelling using different molecular techniques in individual animals. The technique is safe and has no long-term effects on cardiac function or structure.
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http://dx.doi.org/10.1007/s00424-007-0261-z | DOI Listing |
Clin Lung Cancer
December 2024
Department of Thoracic Surgery, Liverpool Heart and Lung Hospital, Liverpool, UK.
Background: To evaluate the real-world surgical and pathological outcomes following neoadjuvant nivolumab in combination with chemotherapy in a multicentre national cohort of patients.
Methods: Retrospective analysis on consecutive patients treated in three tertiary referral hospitals in UK with neoadjuvant chemotherapy and immunotherapy (nivolumab) for stage II-IIIB nonsmall cell lung cancer (March 2023-May 2024). Surgical and pathological outcomes were assessed.
J Cardiothorac Vasc Anesth
December 2024
Department of Medical Science, Surgery and Neurosciences, Cardiothoracic and Vascular Anesthesia and Intensive Care Unit, University Hospital of Siena, Siena, Italy. Electronic address:
Objective: This study investigated if the serratus anterior plane block (SAPB) within a multimodal analgesia scheme would reduce acute post-operative pain and intravenous opioid consumption in patients admitted to the intensive care unit after isolated minimally invasive mitral valve surgery.
Design: Retrospective study.
Setting: Patients were admitted to the intensive care unit (ICU) of the University Hospital of Siena (Italy).
Int J Oral Maxillofac Surg
January 2025
Department of Oral and Maxillofacial Surgery, Universitat Internacional de Catalunya, Sant Cugat del Vallès, Barcelona, Spain; Maxillofacial Institute, Teknon Medical Center, Barcelona, Spain.
A facial appearance of premature aging due to poor bone support of the soft tissues is frequently found in patients with midface hypoplasia. This study was performed to evaluate the changes in the soft tissues of the cheek area in patients subjected to bimaxillary orthognathic surgery. The cheek line angle and length of 27 consecutive patients who underwent bimaxillary surgery, were measured on cone beam computed tomography scans obtained before surgery and at 1 and 12 months after surgery using 3D software.
View Article and Find Full Text PDFJ Dent
January 2025
Clinic of Reconstructive Dentistry, Center for Dental Medicine, University of Zurich, Plattenstrasse 11, CH-8032, Zurich, Switzerland.
Objectives: To evaluate clinical outcomes (restoration survival, technical and biological complications), and patient-reported outcome measures (PROMs) of full mouth rehabilitation with minimally invasive glass-ceramic restorations after up to 12 years of clinical service.
Materials And Methods: Twenty individuals (12 females, 8 males) received full-mouth rehabilitation with minimally invasive tooth-supported glass-ceramic restorations during the years 2009 - 2017 and agreed to participate in a follow-up visit. Full dental and periodontal examinations were completed, and the restorations were evaluated according to United States Public Health Service (USPHS) criteria.
Int J Med Inform
January 2025
University of Coimbra, Faculty of Medicine, Coimbra, Portugal; Department of Gastroenterology, Centro Hospitalar e Universitário de Coimbra, Coimbra, Portugal. Electronic address:
Background: The wireless capsule endoscope (CE) is a valuable diagnostic tool in gastroenterology, offering a safe and minimally invasive visualization of the gastrointestinal tract. One of the few drawbacks identified by the gastroenterology community is the time-consuming task of analyzing CE videos.
Objectives: This article investigates the feasibility of a computer-aided diagnostic method to speed up CE video analysis.
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