Characteristic parameters of erythrocyte aggregation/disaggregation kinetics in blood samples of 200 healthy donors at native and standard hematocrit were studied. Average aggregation parameters for men and women at native hematocrit significantly differed, but these differences disappeared after hematocrit standardization, only maximal amplitude of aggregation (I(0)) at standard hematocrit in women was still less than in men (p<0.001). Though index of strength of largest aggregates (I(a)(2.5)) depended both on sex and age, no significant influence of IgG and IgM concentrations on this parameter was noticed. The obtained results show that suggested method can be used for diagnostics of rheological disorders.
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Background: Due to environmental hypoxia on the high-altitude local residents often exhibit a compensative increase in hemoglobin concentration to maintain the body's oxygen supply. In certain people, the number of red blood cells continues to grow, resulting in high altitude polycythemia (HAPC) which is characterized by headache, disorientation, sleeplessness, and bone discomfort. HAPC is often associated with multiple complications, of which lower extremity arteriosclerosis obliterans (LEASO) is rare.
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January 2025
Clinical Research Centre, University Medical Centre Ljubljana, Zaloška Cesta 2, 1000, Ljubljana, Slovenia.
Premature ovarian insufficiency (POI) is poorly understood, with causes identified in only 25% of cases. Emerging evidence suggests links between trace elements (TEs) and POI. This study is the first to compare concentrations of manganese (Mn), copper (Cu), zinc (Zn), selenium (Se), molybdenum (Mo), arsenic (As), cadmium (Cd), mercury (Hg), and lead (Pb) across urine, serum, and whole blood in women with POI compared to healthy controls (HC), aiming to explore their distribution and potential associations with POI.
View Article and Find Full Text PDFJ Trauma Acute Care Surg
December 2024
From the Department of Surgery, University of Cincinnati, Cincinnati, Ohio.
Background: Red blood cell (RBC) aggregation can be initiated by calcium and tissue factor, which may independently contribute to microvascular and macrovascular thrombosis after injury and transfusion. Previous studies have demonstrated that increased blood storage duration may contribute to thrombotic events. The aims of this study were to first determine the effect of blood product components, age, and hematocrit (HCT) on the aggregability of RBCs, followed by measurement of RBC aggregability in two specific injury models including traumatic brain injury (TBI) and hemorrhagic shock.
View Article and Find Full Text PDFTransfusion
January 2025
Hematology Department, Hospital Universitario Puerta de Hierro-Majadahonda, Majadahonda, Madrid, Spain.
Background: Extracorporeal photopheresis (ECP) product characteristics are not well established. The aim of this study was to compare mononuclear cells (MNCs) collection using the new Amicus blue (AB) In-line ECP system to our standard Off-line ECP system using the Optia apheresis device and the MacoGenic G2 inactivation system (OM).
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Biomicrofluidics
January 2025
School of Mechanical Engineering, Pusan National University, Busan, South Korea.
Monitoring platelet aggregation is crucial for predicting thrombotic diseases and identifying the risk of bleeding or resistance to antiplatelet drugs. This study developed a microfluidic device to measure platelet activation with high sensitivity. By controlling exposure time through repeated reinjections, the device enables the detection of subtle changes in platelet activity influenced by lifestyle factors, such as alcohol consumption.
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