AI Article Synopsis
- Shiga toxin (Stx) is transported inside cells from early endosomes to the Golgi apparatus, where it then moves towards the endoplasmic reticulum and the cytosol.
- The study focused on two proteins, Sorting nexin 1 (SNX1) and Sorting nexin 2 (SNX2), and their roles in the endosomal sorting of Shiga toxin.
- Depleting either SNX1 or SNX2 in Vero cells decreased Stx transport to the Golgi by over 40%, while depleting both led to an 80% reduction, indicating that both proteins are crucial for effective Shiga toxin transport.
Article Abstract
The bacterial toxin Shiga toxin (Stx) is transported retrogradely from early endosomes to the Golgi apparatus on its way to the endoplasmic reticulum (ER) and the cytosol. In this study we explored the functions of the two phosphoinositide binding proteins Sorting nexin 1 (SNX1) and Sorting nexin 2 (SNX2) in endosomal sorting of the toxin. When Vero cells were depleted of either SNX1 or SNX2 by small interfering RNA (siRNA), Stx transport to the trans-Golgi network (TGN) was impaired by > or = 40%, whereas combined depletion of SNX1 and SNX2 gave a total inhibition of approximately 80%. Inhibition of PI(3)P formation by wortmannin resulted in a similar reduction. Thus, although being partly redundant, both SNX1 and SNX2 are required for efficient Stx trafficking to the Golgi apparatus.
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| http://dx.doi.org/10.1016/j.bbrc.2007.04.159 | DOI Listing |
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