The methyl cellulose precipitable (MCP) tannin assay and a modified version of the Somers and Evans color assay were adapted to high-throughput (HTP) analysis. To improve efficiency of the MCP tannin assay, a miniaturized 1 mL format and a HTP format using 96 well plates were developed. The Somers color assay was modified to allow the standardization of pH and ethanol concentrations of wine samples in a simple one-step dilution with a buffer solution, thus removing inconsistencies between wine matrices prior to analysis and allowing for its adaptation to a HTP format. Validation studies showed that all new formats were efficient, and results were reproducible and analogous to the original formats.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/jf063674n | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Drug Development.
Alzheimers Dement
December 2024
Samford University McWhorter School of Pharmacy, Birmingham, AL, USA.
Background: Despite some advances in treatment, a cure for Alzheimer's disease (AD) remains elusive. Disease hallmarks include heightened neuroinflammation and oxidative stress, associated with progressive decline in mobility and cognitive functions. Natural compounds provide a valuable reservoir of novel bioactive substances with therapeutic potential, fewer side effects, and increased affordability.
View Article and Find Full Text PDFPaederia Foetida Linn (Rubiaceae): Chemical Diversity, Phytopharmacological Potential, Quantitative Analysis and Clinical Approaches.
Comb Chem High Throughput Screen
January 2025
KIPS, Shri Shankaracharya Professional University, Bhilai, Chhattisgarh, India.
The discovery of novel counteractive pharmaceuticals, which have recently generated much interest, has played a significant role in the development of drugs derived from herbal medicines or botanical sources. Paederia foetida (P. foetida) is one such example of a role in both traditional and traditional medicine.
View Article and Find Full Text PDFThe assessment of chemical composition and biological activity of faba bean pods as a potential feed additive utilized in piglets nutrition.
Sci Rep
January 2025
Microbiota Lab, Department of Pharmaceutical Biology, Medical University of Warsaw, 1 Banacha St., Warsaw, 02-097, Poland.
The study addresses the utilization of food waste by-products from faba bean (Vicia faba L.) pods (FBP) as an alternative feed supplement to promote sustainable piglet growth by reducing antimicrobial use. Objectives include evaluation of FBP in terms of nutritional components (proximate composition, fibres, minerals), phytochemical composition (total phenols, HPLC-MS profiling), and in vitro biological activities.
View Article and Find Full Text PDFScreening of Phytochemicals as Potential Inhibitors of the JAK/STATs Pathway in Psoriasis.
Assay Drug Dev Technol
January 2025
University Institute of Pharmacy, Pandit Ravishankar Shukla University, Raipur, India.
Phlorotannin-Rich Seaweed Extract Inhibits Influenza Infection.
Viruses
December 2024
Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool L3 5RF, UK.
Seaweed-derived compounds are a renewable resource utilised in the manufacturing and food industry. This study focuses on an enriched seaweed extract (ESE) isolated from The ESE was screened for antiviral activity by plaque reduction assays against influenza A/Puerto Rico/8/1934 H1N1 (PR8), A/X-31 H3N2 (X31) and A/England/195/2009 H1N1 (Eng195), resulting in the complete inhibition of infection. Time of addition assays and FACS analysis were used to help determine the modes of action.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!