AI Article Synopsis
- Poly(DL-lactic-co-glycolic acid) microspheres are created using a double-emulsion method and are designed to deliver the enzyme horseradish peroxidase (HRP), either on their own or as coatings for bioresorbable fibers in tissue regeneration.
- The study examines how the microsphere structure, encapsulation efficiency, and protein release over 12 weeks depend on the copolymer's composition and molecular weight, showing a typical initial burst release followed by slower release mainly governed by diffusion.
- Findings reveal that higher initial molecular weight and increased lactic acid content produce larger, smoother microspheres with reduced burst and total HRP release, indicating that molecular weight has a greater influence than copolymer composition, allowing
Article Abstract
Poly(DL-lactic-co-glycolic acid) microspheres are prepared using a double-emulsion technique and are loaded with the model enzyme horseradish peroxidase (HRP). These microspheres can be used alone or as coatings for bioresorbable fibers that may be used as scaffolds for tissue regeneration applications. The present study focuses on the effect of the copolymer's composition and initial molecular weight on the microsphere structure, encapsulation efficiency, and cumulative protein release for 12 weeks. The release profiles generally exhibits an initial burst effect accompanied by slow release over an extended period of time, during which diffusion rather than degradation controlled HRP release from these structures. An increase in the initial molecular weight or in the copolymer's lactic acid content results in larger microspheres with smoother surfaces, and a decrease in the burst release and in the total HRP release. Molecular weight is found to have a stronger effect than copolymer composition. We demonstrate that it is possible to obtain versatile release profiles, which can be tailored for specific applications by choosing the right initial molecular weight and copolymer composition.
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| http://dx.doi.org/10.1177/0885328207077591 | DOI Listing |
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