A method for the preservation of fungal strains is presented. The cultures are grown on Sabouraud glucose agar in glass ampoules and lyophilized without further processing. By this method the macroscopical morphology of the cultures is preserved, so that these can be used immediately without recultivation as reference cultures. All tested mould and yeast strains remained viable over the six months duration of the experiment, whereas the dermatophyte strains could only partially be recultivated.
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http://dx.doi.org/10.1111/j.1439-0507.1991.tb00639.x | DOI Listing |
Immunol Invest
January 2025
Transplantation Research Institute, Seoul National University Medical Research Center, Seoul, South Korea.
Background: Single-cell RNA sequencing (scRNA-seq) has improved our ability to characterize rare cell populations. In practice, cells from different tissues or donors are simultaneously loaded onto the instrument (multiplexed) at the recommended (standard loading) or higher (superloading) numbers to save time and money. Although cost-effective, superloading can stymie computational analyses owing to high multiplet rates and sample complexity.
View Article and Find Full Text PDFFront Cell Infect Microbiol
January 2025
Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.
Background: is a significant cause of healthcare-associated infections, with rising antimicrobial resistance complicating treatment. This study offers a genomic analysis of , focusing on sequence types (STs), global distribution, antibiotic resistance genes, and virulence factors in its chromosomal and plasmid DNA.
Methods: A total of 19,711 genomes were retrieved from GenBank.
Int J Food Microbiol
January 2025
College of Animal Science and Technology, Yangtze University, Jingzhou 434023, China. Electronic address:
This study investigated endophytic fungi isolated from the medicinal plant Panax notoginseng. Among these, the endophytic fungus SQ3, identified as Chaetomium globosum, was capable of reducing silver ions to form metallic silver nanoparticles. The green-synthesized silver nanoparticles (AgNPs) presented a distinct surface plasmon resonance peak at 424 nm, with particle sizes between 2.
View Article and Find Full Text PDFSci Rep
January 2025
Division of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Faculty of Medicine, Chiang Mai University, 110 Intavaroros Rd., Muaeng, Chiang Mai, 50200, Thailand.
Early diagnosis and appropriate treatment are essential for reducing morbidity and mortality in tuberculous meningitis (TBM). This study aimed to evaluate the diagnostic performance of the Xpert MTB/RIF assay for the diagnosis of TBM in patients with subacute lymphocytic meningitis. This cross-sectional study included 65 cerebrospinal fluid (CSF) specimens from patients at Maharaj Nakorn Chiang Mai University Hospital, Thailand, between January 2015 and March 2016.
View Article and Find Full Text PDFCryobiology
January 2025
Instituto Tecnológico de Chascomús (INTECH). Escuela de Bio y Nanotecnología (UNSAM). Laboratorio de Micología y Cultivo de Hongos Comestibles; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología, Instituto de Microbiología Básica y Aplicada, Laboratorio de Micología Molecular.
Due to their importance in various fields such biotechnology, medicine, food science, preserving microorganisms effectively is essential. This study investigated cryopreservation of the edible fungus Pleurotus pulmonarius and the medicinal mushroom Ganoderma lucidum, for three years at -80°C in sorghum grains without cryoprotectants. We compared viability, growth, and yields with strains maintained on agar media at 5°C.
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