Peridural anaesthesia needles (PDA) do not only differ in calibre or gauge but also vary in the design of the needle tip, which shows a special configuration to place a catheter in the epidural space. A laboratory model was performed to determine the influence of different types of virgin epidural needles concerning their path through tissue. In an additional test run we analysed the influence of a previous use on the path through tissue. We investigated 16 G and 18 G Tuohy needles for epidural anaesthesia and two different 18 G Tuohy needles for combined spinal-epidural anaesthesia. The experiments were performed in a standardised way using a precision drill press in order to make the test independent of the investigator. The test tissue was selected because of its close similarity to human tissue of the lumbar region. Every needle tested revealed a distinct deviation even after a 6 cm passage through the test tissue and regularly deviated away from the concave side of the needle tip. The comparison of the CSE needles tested revealed a significant deviation from the axis due to differences in cross-section and configuration of the needle tip. As expected, the average deviation of PDA needles depended on the diameter. No differences in the degree of deviation for virgin needles compared to previously used needles could be found. Thus, more than one application of the same needle in one patient is considered to be safe as long as the needle is not deformed in any way.
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J Clin Oncol
January 2025
Jefferson Einstein Medical Center, Sidney Kimmel Cancer Center of Thomas Jefferson University, Philadelphia, PA.
Purpose: To evaluate evidence on germline and somatic genomic testing for patients with metastatic prostate cancer and provide recommendations.
Methods: A systematic review by a multidisciplinary panel with patient representation was conducted. The PubMed database was searched from January 2018 to May 2024.
PLoS One
January 2025
Department of Nursing and Podiatry, University of Malaga, Malaga, Spain.
Cellular therapy is a promising treatment option for Peripheral Arterial Disease (PAD). Different cell types can be used to regenerate and repair tissues affected by PAD. Many studies have proposed the use of stem cells, such as mesenchymal stem cells, or even mononuclear cells isolated from peripheral blood or bone marrow, to treat PAD.
View Article and Find Full Text PDFPLoS One
January 2025
AIDS and Cancer Virus Program, Frederick National Laboratory for Cancer Research, Frederick, Maryland, United States of America.
T cell immunotherapy success is dependent on effective levels of antigen receptor expressed at the surface of engineered cells. Efforts to optimize surface expression in T cell receptor (TCR)-based therapeutic approaches include optimization of cellular engineering methods and coding sequences, and reducing the likelihood of exogenous TCR α and β chains mispairing with the endogenous TCR chains. Approaches to promote correct human TCR chain pairing include constant region mutations to create an additional disulfide bond between the two chains, full murinization of the constant region of the TCR α and β sequences, and a minimal set of murine mutations to the TCR α and β constant regions.
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Hematopathology and Transfusion Medicine, University Health Network, Toronto, Ontario, Canada (Xia).
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View Article and Find Full Text PDFEur J Epidemiol
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Department of Dermatology, Erasmus MC Cancer Institute, Rotterdam, The Netherlands.
Early-stage cutaneous melanoma patients generally have a favorable prognosis, yet a significant proportion of metastatic melanoma cases arise from this group, highlighting the need for improved risk stratification using novel prognostic biomarkers. The Dutch Early-Stage Melanoma (D-ESMEL) study introduces a robust, population-based methodology to develop an absolute risk prediction model for stage I/II melanoma, incorporating clinical, imaging, and multi-omics data to identify patients at increased risk for distant metastases. Utilizing the Netherlands Cancer Registry and Dutch Nationwide Pathology Databank, we collected primary tumor samples from early-stage melanoma patients, with and without distant metastases during follow-up.
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