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What are the Thoughts of Women Whose Husbands' Frozen Testicular Sperm Is Thawed for in vitro Fertilization on the Day of Oocyte Retrieval? A Qualitative Study.

Int J Womens Health

January 2025

Nursing Department, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou City, Zhejiang Province, People's Republic of China.

Objective: The psychological experiences will be analyzed to understand the needs and burdens of women on the day of oocyte retrieval when the thawed testicular sperm of their husbands is used for in vitro fertilization, in order to provide a basis for the subsequent formulation of relevant nursing measures.

Methods: This study utilized a descriptive phenomenological research approach. A cohort of 13 women undergoing oocyte retrieval on the day when thawed testicular sperm from their husbands is used for in vitro fertilization at the Reproductive Medicine Center of Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, between August and October 2024, were chosen as participants for this study.

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Reproductive technologies, including sperm cryopreservation, offer conservationists enhanced capacity to genetically manage populations and improve the outcomes of conservation breeding programs (CBPs). Despite this potential, the post-thaw quality of amphibian sperm is highly variable following cryopreservation, and research focused on protocol refinement is needed. The aim of this study was twofold: (1) to investigate the effect of the addition of bovine serum albumin (BSA) to the cryopreservation medium (pre-freeze), and (2) the effect of the addition of caffeine to the activation medium (post-thaw), on post-thaw sperm characteristics in the critically endangered Booroolong frog ().

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AlgaeSperm: Microalgae-Based Soft Magnetic Microrobots for Targeted Tumor Treatment.

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January 2025

School of Mechanical Engineering and Automation, Beihang University, Beijing, 100191, China.

Magnetic microrobots are significant platforms for targeted drug delivery, among which sperm-inspired types have attracted much attention due to their flexible undulation. However, mass production of sperm-like soft magnetic microrobots with high-speed propulsion is still challenging due to the need of more reasonable structure design and facile fabrication. Herein, a novel strategy is proposed for large-scale preparation of microalgae-based soft microrobots with a fully magnetic head-to-tail structure, called AlgaeSperm with robust propulsion and chemo-photothermal performance.

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Swimming by Spinning: Spinning-Top Type Rotations Regularize Sperm Swimming Into Persistently Progressive Paths in 3D.

Adv Sci (Weinh)

December 2024

School of Engineering Mathematics and Technology & Bristol Robotics Laboratory, University of Bristol, Bristol, BS8 1UB, UK.

Sperm swimming is essential for reproduction, with movement strategies adapted to specific environments. Sperm navigate by modulating the symmetry of their flagellar beating, but how they swim forward with asymmetrical beats remains unclear. Current methods lack the ability to robustly detect the flagellar symmetry state in free-swimming spermatozoa, despite its importance in understanding sperm motility.

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S-adenosylmethionine and S-adenosyl-L-homocysteine metabolism is involved in the sperm motility and in vitro fertility rate in mouse.

Biochem Biophys Res Commun

December 2024

Department of Cellular Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan; Neutron Therapy Research Center, Okayama University, Okayama, Japan. Electronic address:

Increased fragmentation of sperm DNA has been implicated in male infertility. Folate deficiency results in impaired methionine synthesis, depletion of S-adenosylmethionine (SAM) levels, an increase in S-adenosyl-l-homocysteine (SAH) levels, and increased DNA fragmentation. Disruption of the dynamic balance between SAM and SAH may also contribute, although the details of this process are not yet fully understood.

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