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Is fine morphology of the human sperm nuclei affected by in vitro incubation at 37 degrees C? | LitMetric

Is fine morphology of the human sperm nuclei affected by in vitro incubation at 37 degrees C?

Fertil Steril

Male Fertility Laboratory, The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan, Israel.

Published: December 2007

Objective: Incubation of ejaculated spermatozoa at 37 degrees C is recommended for IVF-intracytoplasmic sperm injection. Preselection of sperm cells with morphologically normal nuclei before microinjection, adapted in our laboratory, is usually a time-consuming procedure. Therefore, we aimed to verify whether incubation at 37 degrees C could affect the morphologic integrity of sperm nuclei.

Design: Time-kinetics studies testing fine morphology of the sperm nuclei upon prolonged in vitro incubation.

Setting: Male Fertility Laboratory at Bar-Ilan University, Ramat Gan, Israel.

Patient(s): Forty-two males selected at random, who were referred for sperm preselection before ICSI.

Main Outcome Measure(s): Morphologic integrity of the sperm nuclei, obtained by motile sperm organelle morphology examination.

Result(s): After 2 hours of incubation at 37 degrees C a significant decrease occurred in the morphologic integrity of the sperm nuclei, compared with the initial state (4.7% +/- 2.8% vs. 6.8% +/- 3.5%, t = 3.2, P
Conclusion(s): Prolonged (>or=2 hours) sperm manipulations for assisted reproduction therapy should be performed at 21 degrees C rather than 37 degrees C.

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Source
http://dx.doi.org/10.1016/j.fertnstert.2007.01.069DOI Listing

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