In a comparison of the Bactec system and the lysis concentration procedure in the isolation of Brucella species in 54 patients the recovery rate was similar (60% and 55%, respectively). However, the recovery time was significantly shorter with the lysis concentration method than with the Bactec system (3.5 days versus 14 days). The lysis concentration procedure for the culture of Brucella is simple, inexpensive and reliable, and produces results for the clinician relatively quickly.
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http://dx.doi.org/10.1007/BF01975817 | DOI Listing |
Biosensors (Basel)
January 2025
Biomedical Engineering Program, Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA 23508, USA.
Cell lysis is the starting step of many biomedical assays. Electric field-based cell lysis is widely used in many applications, including point-of-care (POC) applications, because it provides an easy one-step solution. Many electric field-based lysis methods utilize micro-electrodes to apply short electric pulses across cells.
View Article and Find Full Text PDFBackground: Uncertainty about optimal tranexamic acid (TXA) dosage has led to significant practice variation in hip arthroplasty. We aimed to identify the optimal i.v.
View Article and Find Full Text PDFZhongguo Xue Xi Chong Bing Fang Zhi Za Zhi
December 2024
Lixiahe Institute of Agricultural Sciences in Jiangsu Province; National Experimental Station of Yangzhou for Agricultural Microbiology, Yangzhou, Jiangsu 225007, China.
Objective: To investigate the physiological characteristics of subspecies (Bti) with double mutations of and genes and to assess the activity of Bti against larvae of under different external factors, so as to provide the theoretical evidence for the use of engineered bacteria of Bti for effective mosquito control.
Methods: wild-type strain Bt-59 and Bt-59 strain with mutation [Bt-59 (Δ)] were cultured in nutrient broth media for 24 hours, and Bt-59 strains with mutation [Bt-59 (Δ)] and double mutations of and [Bt-59 (Δ)] were cultured in nutrient broth media for 48 hours. Then, 5 μL of culture media were transferred to glass sides, and cell morphology and mother cell lysis were observed under an optical microscope.
Adv Sci (Weinh)
January 2025
Department of Electrical Engineering, Kwangwoon University, Seoul, 01897, Republic of Korea.
During the pandemic surge, including SARS-CoV-2 and influenza, pooling samples emerged as an efficient strategy to identify infected individuals in large groups. While pooling enhances RT-PCR throughput, reducing costs and resources, it dilutes positive samples with negative ones, lowering sensitivity and increasing false negatives. This study proposes a new method to address the trade-off between pool sizes and RT-PCR accuracy.
View Article and Find Full Text PDFRSC Adv
January 2025
Department of Chemistry, Jamia Millia Islamia Jamia Nagar New Delhi-110025 India
Antibiotic-resistant bacteria are a serious global health threat, making infections harder to treat and increasing medical costs and mortality rates. To combat resistant bacterial strains, a series of compounds (QS1-12) were synthesized with an excellent yield of 85-92%. Initial assessments of these analogues as potential antibacterial agents were conducted through a preliminary screening against a panel of diverse bacterial strains.
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