AI Article Synopsis
- A new method for detecting urinary bile acids using electrospray ionization mass spectrometry is presented, which is simple, sensitive, and accurate.
- The method specifically targets sulfated tauro- and glycodihydroxycholic acids, which generate unique negative ions in urine samples.
- This technique is particularly useful for screening biliary atresia in newborns, as it helps identify elevated levels of sulfated taurodihydroxycholic acid in patients with cholestasis.
Article Abstract
Here we report a simple, sensitive, and accurate method for detecting urinary sulfated tauro- and glyco-bile acids that uses electrospray ionization mass spectrometry. The sulfated tauro- and glycodihydroxycholic acids mainly generated [M-2H](2-) negative ions at m/z 288.6 and m/z 263.6, respectively. These doubly charged ions appeared primarily in samples prepared from the urine of patients with cholestasis and were detected quantitatively. Cholestatic jaundice is the primary clinical sign of biliary atresia. The measurement of doubly charged negative ions, especially of sulfated taurodihydroxycholic acid (principally taurochenodeoxycholate-3-sulfate), is a useful screening modality for biliary atresia in neonates.
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| http://dx.doi.org/10.1016/j.jchromb.2007.04.010 | DOI Listing |
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