A thioredoxin (Trx) gene was cloned from the silkworm, Bombyx mori. The B. mori Trx (BmTrx) cDNA contains an open reading frame of 318 bp encoding 106 amino acid residues with a conserved active site (CGPC). Northern blot analysis revealed the presence of BmTrx transcripts in all tissues examined. The cDNA encoding BmTrx was expressed as a 12-kDa polypeptide in baculovirus-infected insect Sf9 cells. The recombinant BmTrx proved to be biologically active, using an insulin reduction assay, and was also able to activate thioredoxin peroxidase from B. mori. When H2O2 or paraquat was injected into the body cavity of B. mori larvae, BmTrx mRNA expression was upregulated in the fat body tissue. In addition, the expression levels of BmTrx mRNA in the fat body were greatly increased when B. mori larvae were exposed to low or high temperatures, or injected with microorganisms. These results suggest that BmTrx possibly protects against oxidative stress caused by extreme temperatures and microbial infection as well as by intracellularly generated reactive oxygen species during metabolism.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.cbpb.2007.03.012 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Conserved Nuclear Localization Signal in NS2 Protein of Bombyx Mori Bidensovirus: A Potential Invertebrate ssDNA Virus Trait.
Viruses
January 2025
School of Life Sciences, Jiangsu University, Zhenjiang 212013, China.
Bombyx mori bidensovirus (BmBDV), a significant pathogen in the sericulture industry, holds a unique taxonomic position due to its distinct segmented single-stranded DNA (ssDNA) genome and the presence of a self-encoding DNA polymerase. However, the functions of viral non-structural proteins, such as NS2, remain unknown. This protein is hypothesized to play a role in viral replication and pathogenesis.
View Article and Find Full Text PDFEfficient and Rapid Microfluidics Production of Bio-Inspired Nanoparticles Derived from Silkworm for Enhanced Breast Cancer Treatment.
Pharmaceutics
January 2025
School of Medicine and Population Health, The University of Sheffield, Barber House, Sheffield S10 2HQ, UK.
: In the quest for sustainable and biocompatible materials, silk fibroin (SF), derived from natural silk, has emerged as a promising candidate for nanoparticle production. This study aimed to fabricate silk fibroin particles (SFPs) using a novel swirl mixer previously presented by our group, evaluating their characteristics and suitability for drug delivery applications, including magnetic nanoparticles and dual-drug encapsulation with curcumin (CUR) and 5-fluorouracil (5-FU). : SFPs were fabricated via microfluidics-assisted desolvation using a swirl mixer, ensuring precise mixing kinetics.
View Article and Find Full Text PDFEffects of Novaluron Exposure on the Oviposition and Expression of Ovarian Development Related Genes in Silkworm, (Lepidoptera: Bombycidae).
Insects
December 2024
Jiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, China.
(Lepidoptera: Bombycidae) is an important economic insect, which mainly feeds on mulberry leaves and is widely used in many research fields. The growth and development of silkworm larvae are easily affected by the use of chemical insecticides such as novaluron, a benzoylurea insecticide. However, the effect of novaluron exposure on the reproduction of silkworms has not yet been studied.
View Article and Find Full Text PDFGenome-Wide Characterization of Extrachromosomal Circular DNA in the Midgut of BmCPV-Infected Silkworms and Its Potential Role in Antiviral Responses.
Int J Mol Sci
January 2025
School of Life Sciences, Soochow University, Suzhou 215123, China.
Extrachromosomal circular DNAs (eccDNAs) has been found to be widespread and functional in various organisms. However, comparative analyses of pre- and post-infection of virus are rarely known. Herein, we investigated the changes in expression patterns of eccDNA following infection with cytoplasmic polyhedrosis virus (BmCPV) and explore the role of eccDNA in viral infection.
View Article and Find Full Text PDFNatural Protein Films from Textile Waste for Wound Healing and Wound Dressing Applications.
J Funct Biomater
January 2025
National Research Council, Institute for Organic Synthesis and Photoreactivity (CNR-ISOF), Via P. Gobetti 101, 40129 Bologna, Italy.
In recent years, several studies have focused on the development of sustainable, biocompatible, and biodegradable films with potential applications in wound healing and wound dressing systems. Natural macromolecules, particularly proteins, have emerged as attractive alternatives to synthetic polymers due to their biocompatibility, biodegradability, low immunogenicity, and adaptability. Among these proteins, keratin, extracted from waste wool, and fibroin, derived from cocoons, exhibit exceptional properties such as mechanical strength, cell adhesion capabilities, and suitability for various fabrication methods.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!