AI Article Synopsis
- Oxidative DNA damage is a critical factor in various severe diseases, including cancer and heart disease, and also affects common health issues.
- Research has revealed mechanisms of DNA oxidation, notably through nickel(II) which causes irregular production of 8-oxoguanine, a DNA damage marker, from both free guanine and DNA.
- Further studies have identified additional oxidized forms of guanine, such as oxidized-guanidinohydantoin and guanidinohydantoin, depending on the pH conditions during the experiments.
Article Abstract
Oxidative DNA damage is one of the most important and most studied mechanisms of disease. It has been associated with a range of terminal diseases such as cancer, heart disease, hepatitis, and HIV, as well as with a variety of everyday ailments. There are various mechanisms by which this type of DNA damage can be initiated, through radiation and chemical oxidation, among others; however, these mechanisms have yet to be fully elucidated. A HPLC-UV-EC study of the oxidation of DNA mediated by nickel(II) obtained results that show an erratic, almost oscillatory formation of 8-oxoguanine (8-oxoG) from free guanine and from guanine in DNA. Sporadic 8-oxoG concentrations were also observed when 8-oxoG alone was subjected to these conditions. A HPLC-MS/MS study showed the formation of oxidised-guanidinohydantoin (oxGH) from free guanine at pH 11, and the formation of guanidinohydantoin (GH) from DNA at pH 5.5.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.freeradbiomed.2007.02.025 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!